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SPHKAP scFv [L131/27]

Plasmid

#190513

Purpose

Mammalian Expression of SPHKAP scFV. Derived from hybridoma L131/27.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

SPHKAP (Mus musculus) recombinant scFV (Sphkap Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Pancortin scFv [K96/7]

Plasmid

#190497

Purpose

Mammalian Expression of Pancortin scFV. Derived from hybridoma K96/7.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pancortin (Homo sapiens) recombinant scFV (OLFM1 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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QKI-7 scFv [N183/15]

Plasmid

#190536

Purpose

Mammalian Expression of QKI-7 scFV. Derived from hybridoma N183/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

QKI-7 (Mus musculus) recombinant scFV (Qki Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Shank2 scFv [N23B/6]

Plasmid

#190543

Purpose

Mammalian Expression of Shank2 scFV. Derived from hybridoma N23B/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Shank2 (Rattus norvegicus) recombinant scFV (Shank2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Rufy3 scFv [N483/84]

Plasmid

#190559

Purpose

Mammalian Expression of Rufy3 scFV. Derived from hybridoma N483/84.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Rufy3 (Mus musculus) recombinant scFV (Rufy3 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-SAPAP1/2 [N238/31R]

Plasmid

#182110

Purpose

Mammalian Expression Plasmid of anti-SAPAP1/2 (). Derived from hybridoma N238/31.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-SAPAP1/2 () recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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CIRC

Plasmid

#186343

Purpose

Contains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)

Depositor

Giovanni Blandino

Article

Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

Insert

VEGFA (VEGFA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-HsSyx3

Plasmid

#179289

Purpose

E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + GFP + TEV protease cleavage site

Depositor

Debra Hansen

Article

Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

Insert

Syx

Use

Tags

His6 + GFP + TEV protease cleavage site

Expression

Bacterial

Mutation

residues 393-792 from accession number NP_0010361…

Promoter

T7

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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px335 Slc1a3 nickase 1

Plasmid

#129410

Purpose

Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

Depositor

Walker Jackson

Article

Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

Insert

sgRNA directing Cas9n to the proximity of Slc1a3 STOP codon (Slc1a3 Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX2T-TEV CHMP1B 4-178

Plasmid

#108284

Purpose

Expresses residues 4-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-279

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Use

Tags

GST

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 179-199

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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