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pSELIS-RamR

Plasmid

#202631

Purpose

Enables dual growth and fluorescence based enrichment of RamR biosensors

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

Sh ble

Use

Synthetic Biology

Expression

Bacterial

Promoter

Lambda promoter

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pJB007

Plasmid

#98995

Purpose

Express FtsI tagged with tagRFP-t in E coli

Depositor

Jie Xiao

Article

Yang et al Science. 2017 Feb 17;355(6326):744-747.

Insert

FtsI

Tags

TagRFP-T

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pRPF185

Plasmid

#106367

Purpose

C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

Depositor

Robert Fagan, Neil Fairweather

Article

Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

Insert

Term(fdx)-Ptet-gusA-Term(slpA) (gusA )

Use

E. coli - c. difficile shuttle vector

Mutation

codon optimised for C. difficile

Promoter

Ptet

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pBzCas13b

Plasmid

#164862

Purpose

Inducible expression of BzCas13b nuclease in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

Bergeyella zoohelcum Cas13b

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-ORF-mScarlet

Plasmid

#217453

Purpose

Gateway destination vector with C-terminal mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Type

Empty backbone

Use

Gateway destination vector

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-mScarlet-ORF

Plasmid

#217454

Purpose

Gateway destination vector with N-terminal mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Type

Empty backbone

Use

Gateway destination vector

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBIG2ABC-eIF3Δbgi

Plasmid

#240043

Purpose

Human eIF3k, eIF3l, eIF3e, eIF3f, eIF3h, eIF3d, eIF3m, eIF3a and eIF3c expression in insect cells for purification

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3k, l, e, f, h, d, m, a, c

Tags

eIF3a: TEV_3xFLAG, eIF3c: 3C_STREP II, eIF3d: HA

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCN38-yfp

Plasmid

#227637

Purpose

Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-48-3xFLAG

Plasmid

#130675

Purpose

Expresses full-length human SMCHD1 with a C-terminal 3x FLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET21-HIS-Tol2

Plasmid

#112486

Purpose

functional recombinant Tol2 protein from E.coli

Depositor

Stephen Ekker

Article

Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

Insert

Tol2 Transposase (tpase Synthetic)

Tags

HIS/6x

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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