161,152 results

1. pRSET-RCaMP1h

   Plasmid

   #42874

   Purpose

   a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

   Depositor

   Loren Looger

   Article

   Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

   Insert

   Red Fluorescent Calcium binding Protein
   

   Use

   Tags

   EK (Enterokinase) Recognition Site, His Tag (6x),…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #180280

   Purpose

   The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (backbone) and BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FLAG-LKB1

   Plasmid

   #8590

   Depositor

   Lewis Cantley

   Article

   Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

   Insert

   LKB1 (STK11 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

   Plasmid

   #85008

   Purpose

   For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   EGFP-ires-tTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. tol2-mpeg1-mcherry

   Plasmid

   #58935

   Purpose

   labels macrophages with mcherry

   Depositor

   Anna Huttenlocher

   Insert

   mpeg1-mcherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   mpeg1

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAGGS-EGFP-NLS-3XFLAG-V5

   Plasmid

   #196090

   Purpose

   Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   GFP
   

   Use

   Tags

   C-terminal 3xFLAG and C-terminal V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAGGs

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-UVRAG

   Plasmid

   #86743

   Purpose

   N-terminal mCherry-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

   Insert

   human UVRAG (UVRAG Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-mCherry

   Plasmid

   #197420

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. p426MET25_sfpHluorin (MRV55)

   Plasmid

   #115697

   Purpose

   superfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)

   Depositor

   Eckhard Boles

   Article

   Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

   Insert

   sfpHluorin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   for introduced mutations see publication

   Promoter

   MET25

   Available since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB533-42B3mutAC2-sfGFP

    Plasmid

    #186777

    Purpose

    RNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cells

    Depositor

    Hiroshi Kimura

    Article

    Uchino et al J Cell Biol. 2022 Feb 7;221(2). pii: 212

    Insert

    42B3mutAC2-scFv
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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