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Addgene

We narrowed to 2,493 results for: ERK

Showing: 2441 - 2480 of 2493 results
  1. EFNB1-bio-His

    Plasmid
    #51749
    Purpose
    Expresses full-length Ephrin-B1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Insert
    EFNB1 (EFNB1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. piRFP670-N1-GalT

    Plasmid
    #87325
    Purpose
    To express GalT-iRFP670 in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
    Depositor
    Insert
    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    Use
    Tags
    iRFP670
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
  3. pET-His6-SUMO-MproC145A

    Plasmid
    #172653
    Purpose
    Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO
    Depositor
    Insert
    SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHis Tag and SUMO
    Expression
    Bacterial
    Mutation
    Catalytic Cysteine 145 mutated to Alanine
    Promoter
    T7
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA4/TO-bio-myc-NES-EGFP

    Plasmid
    #112712
    Purpose
    For tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cells
    Depositor
    Insert
    EGFP
    Use
    Tags
    HIV Rev nuclear localization signal (NES), biotin…
    Expression
    Mammalian
    Mutation
    Nucleotide sequence optimized for synthetic gene …
    Promoter
    CMV with Tet repressor binding sites
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. Lyn-FLINC-EKAR1(TA)

    Plasmid
    #87708
    Purpose
    Negative-control mutant of Lyn-FLINC-EKAR1.
    Depositor
    Insert
    Lyn-FLINC-EKAR1(TA)
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA3-bio-myc-mCE 2-210

    Plasmid
    #112715
    Purpose
    Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells
    Depositor
    Insert
    Rngtt (Rngtt Mouse)
    Use
    Tags
    biotinylation signal peptide (Tagwerker et al. 20…
    Expression
    Mammalian
    Mutation
    Silent mutation at Arg 132 (CGT to CGC), deleted …
    Promoter
    CMV
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA3-bio-myc-mCE 211-597

    Plasmid
    #112716
    Purpose
    Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 211-597 in mammalian cells
    Depositor
    Insert
    Rngtt (Rngtt Mouse)
    Use
    Tags
    biotinylation signal peptide (Tagwerker et al. 20…
    Expression
    Mammalian
    Mutation
    deleted amino acids 2-210 (triphosphatase domain)
    Promoter
    CMV
    Available since
    Sept. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pCEP4-myc-EFNB2 (D62Q-Q130L-V167L)

    Plasmid
    #200977
    Purpose
    Mammalian expression plasmid for myc-tagged EFNB2 (D62Q-Q130L-V167L specificity mutant)
    Depositor
    Insert
    EFNB2 (EFNB2 Human)
    Use
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    D62Q-Q130L-V167L (increase specificity towards he…
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 2441 - 2480 of 2493 results