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Plasmid#68581PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only
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p221z-CAS9p-TagRFP-t35s
Plasmid#118386PurposeEntry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertCAS9p-TagRFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCR4-HBGP::mCherry-SI-HBGP::eGFP-H11
Plasmid#216394Purposedual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct (human beta-globin promoter) into the H11 locus (separated by a synthetic insulator)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterHuman beta-globinAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCBC-MT2T3
Plasmid#50594PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT2, gRNA scaffold, TaU3t plus, U6-26p, T3
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-Tnos
Plasmid#211840PurposePlant transient expression cassette for the catalytically inactive E3-DART ligase under a UBQ10 promoter.DepositorInsertLRR-NEL
UseSynthetic BiologyTagsHA and mCherryExpressionPlantMutationPromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
ePPE-spG
Plasmid#183096PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertNls-nspGCas9-NC-nls-MLV-Nls
UseCRISPRTagsExpressionPlantMutationD10A in SpGCas9PromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0101
Plasmid#90998PurposeModule A, Promoter: 35S, Gene: AtCas9, Terminator: HSPDepositorInsertAtCas9
UseCRISPRTagsExpressionMutationPromoter35SAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:(MCS)-S11
Plasmid#108179PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0501
Plasmid#91011PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-AtCas9, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9
UseCRISPRTagsExpressionMutationPromoter35SAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGE6
Plasmid#79476PurposesgRNA shuttle vector; module 1EDepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-Tnos
Plasmid#211843PurposePlant transient expression cassette for the E3-DART ligase under a UBQ10 promoter.DepositorInsertLRR-NEL
UseSynthetic BiologyTagsHA and mCherryExpressionPlantMutationC492APromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ152
Plasmid#69303PurposeGateway entry vector with pco-dCas9-VP64DepositorInsertpco-dCas9-VP64 (Plant codon-optimized)
UseCRISPRTagsExpressionMutationVP64 activator fusion; NLS fusionPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0103
Plasmid#91000PurposeModule A, Promoter: CmYLCV, Gene: AtCas9, Terminator: HSPDepositorInsertAtCas9
UseCRISPRTagsExpressionMutationPromoterCmYLCVAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos
Plasmid#211841PurposePlant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantMutationPromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPO5
Plasmid#209987PurposeContains Level 0* Part: replication origin (256rep) for the construction of Level 2 plasmidsDepositorInsertreplication origin (256_rep)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPP7
Plasmid#209969PurposeContains Level 0 Part: autoinducible Promoter (PfabZ) for the construction of Level 1 plasmidsDepositorInsertPromoter (P_fabZ)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPP8
Plasmid#209970PurposeContains Level 0 Part: autoinducible Promoter (PgadB) for the construction of Level 1 plasmidsDepositorInsertPromoter (P_gadB)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPP9
Plasmid#209971PurposeContains Level 0 Part: constitutive Promoter (PldhD) for the construction of Level 1 plasmidsDepositorInsertPromoter (P_ldhD)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCBC-MT3T4
Plasmid#50595PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT3, gRNA scaffold, U6-26t plus, OsU3p, T4
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3003
Plasmid#91095PurposeModule C, Promoter: CmYLCV, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedTagsExpressionMutationPromoterCmYLCVAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3012
Plasmid#91099PurposeModule C, Promoter: PvUbi1, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedTagsExpressionMutationPromoterPvUbi1Available sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUB-Cas9-@GL1
Plasmid#86779PurposeDisruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9DepositorInsertsgRNA against GLABROUS1
UseSynthetic BiologyTagsExpressionPlantMutationPromoterArabidopsis U6-26 promoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3001
Plasmid#91094PurposeModule C, Promoter: 35S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedTagsExpressionMutationPromoter35SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1910
Plasmid#91041PurposeModule A, Promoter: ZmUbi, Gene: TREX2-P2A-TaCas9, Terminator: HSPDepositorInsertTREX2-P2A-TaCas9
UseCRISPRTagsExpressionMutationPromoterZmUbiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30a-FluB-NP
Plasmid#165093Purposeprotein expression in E. coliDepositorInsertInfluenza B virus(B/Georgia/05/2019) nucleocapsid protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMOD_C3006
Plasmid#91096PurposeModule C, Promoter: FMV 34S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedTagsExpressionMutationPromoterFMV 34SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMP4657
Plasmid#107773PurposeExpression of EGFPDepositorInsertpLacZ-EGFP in pME6010
UseTagspLacZ-EGFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMP4639
Plasmid#107771PurposeExpression of EYFPDepositorInsertpLacZ-EYFP in pME6010
UseTagspLacZ-EYFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:GFP1–9
Plasmid#108187PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components (GFP1-9) in plantsDepositorInsertGFP1-9
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLPO3
Plasmid#209977PurposeContains Level 0 Part: replication origin (pAMBeta1) for the construction of Level 1 plasmidsDepositorInsertreplication origin (pAMBeta1)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3010
Plasmid#91098PurposeModule C, Promoter: ZmUbi, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedTagsExpressionMutationPromoterZmUbiAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Px
Plasmid#183170PurposeConstruct for transient expression of peroxisomes-targeted mCherry in plants.DepositorInsertmCherry-Px
UseTags10 aa peroxisome targeting signal 1 (IHHPRELSRL) …ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0902
Plasmid#91026PurposeModule A, Promoter: AtUbi10, Gene: TREX2-P2A-AtCas9, Terminator: HSPDepositorInsertTREX2-P2A-AtCas9
UseCRISPRTagsExpressionMutationPromoterAtUbi10Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290
Plasmid#129670PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b
UseCRISPR; Gateway compatible aaccas12b entry cloneTagsExpressionPlantMutationAacCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMP4518
Plasmid#107778PurposeExpression of EYFPDepositorInsertpLacZ-EYFP in pBBR1-mcs5
UseTagspLacZ-EYFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB508-cTP-sfGFP
Plasmid#213863PurposeBinary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)DepositorInsertPlastid transit peptide fused sfGFP
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFLIPglu-2uDelta13
Plasmid#12990DepositorInsertFLIPglu F16F D152A Delta13
UsePlant nanosensorTagsECFP and EYFPExpressionPlantMutationD152A mutation in binding proteinPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292
Plasmid#129672PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b
UseCRISPR; Gateway compatible aacas12b entry cloneTagsExpressionPlantMutationAaCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19/Pd-mChe
Plasmid#183172PurposeConstruct for transient expression of plasmodesmata-targeted mCherry in plants.DepositorInsertPd-mCherry
UseTagsAtPDCB1, A. thaliana PLASMODESMATA CALLOSE-BINDIN…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFLIPglu-600uDelta13
Plasmid#12991DepositorInsertFLIPglu F16A Delta13
UsePlant nanosensorTagsECFP and EYFPExpressionPlantMutationF16A in binding proteinPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:(MCS)-3xHA-S11
Plasmid#108180PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:S10-(MCS)-3xHA
Plasmid#108178PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-Cas12j2
Plasmid#173924PurposeGateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLSDepositorInsertNLS-Cas12j2-NLS
UseCRISPRTagsNLSExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM37443
Plasmid#153218PurposeLevel 2 binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMP4516
Plasmid#107775PurposeExpression of ECFPDepositorInsertpLacZ-ECFP in pBBR1-mcs5
UseTagspLacZ-ECFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1
Plasmid#68228PurposeGoldenBraid level 1 Alpha plant expression vector.DepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Cs
Plasmid#183171PurposeConstruct for transient expression of cytoskeleton-targeted mCherry in plants.DepositorInsertmCherry-Cs
UseTags363 aa of A. thaliana FIMBRIN 1 (AtFIM1)ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIK-GW
Plasmid#126556PurposeBroad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection, Gateway cloning compatibleDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB326
Plasmid#68654PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterNo PromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE8
Plasmid#79463PurposesgRNA shuttle vector; module 2EDepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only