We narrowed to 11,674 results for: NSI

1. pcDNA4/TO-2xStrep-UL32 H581A

   Plasmid

   #162648

   Purpose

   Expresses N-terminal 2x-Strep-tagged UL32 H581A in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   UL32
   

   Tags

   N-terminal 2xStrep-Tag II

   Expression

   Mammalian

   Mutation

   Amino acid H581 mutated to alanine

   Promoter

   CMV

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4/TO-2xStrep-UL32 C155A

   Plasmid

   #162649

   Purpose

   Expresses N-terminal 2x-Strep-tagged UL32 C155A in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   UL32
   

   Tags

   N-terminal 2xStrep-Tag II

   Expression

   Mammalian

   Mutation

   Amino acid C155 mutated to alanine

   Promoter

   CMV

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4/TO-2xStrep-ORF68 C52A

   Plasmid

   #162630

   Purpose

   Expresses N-terminal 2x-Strep-tagged ORF68 C52A in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Tags

   N-terminal 2xStrep-Tag II

   Expression

   Mammalian

   Mutation

   Amino acid C52 mutated to alanine

   Promoter

   CMV

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA4/TO-2xStrep-ORF68 C373A

   Plasmid

   #162631

   Purpose

   Expresses N-terminal 2x-Strep-tagged ORF68 C373A in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Tags

   N-terminal 2xStrep-Tag II

   Expression

   Mammalian

   Mutation

   Amino acid C373 mutated to alanine

   Promoter

   CMV

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4/TO-2xStrep-ORF68 C191A/C192A

   Plasmid

   #162632

   Purpose

   Expresses N-terminal 2x-Strep-tagged ORF68 C191A/C192A in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Tags

   N-terminal 2xStrep-Tag II

   Expression

   Mammalian

   Mutation

   Amino acids C191 and C192 mutated to alanine

   Promoter

   CMV

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-Synapsin-mTurquoise2-optop38i5-WPRE

   Plasmid

   #159972

   Purpose

   optogenetic p38 inhibitor with RET to LOV2 to increase sensitivity

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   mTurquoise2-optop38i5
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-Synapsin-mTurquoise2-optop38i3-WPRE

   Plasmid

   #159971

   Purpose

   optogenetic p38 inhibitor with RET to LOV2 to increase sensitivity

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   mTurquoise2-optop38i3
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-Synapsin-mTurquoise2-optoJNKi-WPRE

   Plasmid

   #159966

   Purpose

   optogenetic JNK inhibitor with increased RET to LOV2 to increase actuation sensitivity

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   mTurquoise2-optoJNKi
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-Avi-Coh (E154C)-HIS

   Plasmid

   #153447

   Purpose

   E. coli expression of Rc. CohE construct with E154C mutation.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.CohE
   

   Tags

   6xHis and Avi-tag

   Expression

   Bacterial

   Mutation

   E154C mutation on CohE

   Promoter

   T7 promoter

   Available Since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGENE B::AtHAP2-V5

    Plasmid

    #132959

    Purpose

    Express AtHAP2 full length protein in mammalian cells

    Depositor

    Benjamin Podbilewicz

    Article

    Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

    Insert

    AtHAP2::V5
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. UL79(E48A_L49A)-3xFLAG pCDNA4

    Plasmid

    #129759

    Purpose

    Expresses CMV UL79 (E48A_L49A) with Cterm 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    UL79 (UL79 Human Cytomegalovirus (HHV-5))
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Glutamic acid 48 to Alanine and Leucine 4…

    Promoter

    CMV

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MtMscL delta49

    Plasmid

    #118342

    Purpose

    M. tuberculosis MscL (delta49) in pET19b

    Depositor

    Douglas Rees

    Article

    Herrera et al Sci Rep. 2018 Oct 1;8(1):14566. doi: 10.

    Insert

    MscL (mscL Mycobacterium tuberculosis)
    

    Tags

    6x His Tag and Thrombin Cleavage site

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 102 to Stop Codon

    Promoter

    T7 Promoter

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMCh-1469

    Plasmid

    #82419

    Purpose

    Plasmid for expression of NHA-tagged Nematostella vectensis GW182 in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182
    

    Tags

    NHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMCh-1547

    Plasmid

    #82434

    Purpose

    Plasmid for expression of HA-tagged Nematostella vectensis AGO1 in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvAGO1
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMCh-1548

    Plasmid

    #82435

    Purpose

    Plasmid for expression of HA-tagged Nematostella vectensis AGO2 in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvAGO2
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYSD061

    Plasmid

    #212915

    Purpose

    Encodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    SUC2 (SUC2 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. TfR-pHuji

    Plasmid

    #61505

    Purpose

    Human transferrin receptor tagged with pH sensitive red fluorescent protein pHuji

    Depositor

    David Perrais

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    Transferrin receptor (TFRC Human)
    

    Tags

    pHuji

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET19b-EcMscL-TEV-mEGFP

    Plasmid

    #165097

    Purpose

    Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.

    Depositor

    Neha Kamat

    Article

    Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p

    Insert

    E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
    

    Use

    Synthetic Biology

    Tags

    monomeric Enhanced Green Fluorescent Protein

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIRE4-Azi

    Plasmid

    #105829

    Purpose

    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

    Depositor

    Irene Coin

    Article

    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

    Inserts

    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    

    Expression

    Mammalian

    Mutation

    (Y37L, D182S, F183M, L186A, D265R)TyrRS

    Promoter

    PGK and U6

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1(+)-NEMOb

    Plasmid

    #189934

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOb
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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