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Addgene

We narrowed to 9,250 results for: SUB;

Showing: 1221 - 1240 of 9250 results
  1. pJK650

    Plasmid
    #72400
    Purpose
    Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)
    Depositor
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pTU1-D-recJD

    Plasmid
    #112784
    Purpose
    Downstream homology arm, RecJ is a ssDNA specific exonuclease, involved in genome repair and maintenance
    Depositor
    Insert
    recJD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. His8:MBP-tev-Gly-eK-Flv

    Plasmid
    #83383
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. pD(P+T)_phN

    Plasmid
    #223858
    Purpose
    4G-cloning donor for insect promoter and terminator, N-terminal tag added
    Depositor
    Insert
    polyhedrin promoter and SV40 polyA
    Use
    Golden-gate donor
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pD(P+T)_phC

    Plasmid
    #223859
    Purpose
    4G-cloning donor for insect promoter and terminator, C-terminal tag added
    Depositor
    Insert
    polyhedrin promoter and SV40 polyA
    Use
    Golden-gate donor
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pIND4-RC-C2-MmXYRS

    Plasmid
    #222744
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for incorporating haloalkane crosslinker
    Depositor
    Inserts
    puhA
    pufL
    pufM
    MmXYRS
    Mm Pyl tRNA
    Use
    Tags
    6xHis
    Expression
    Mutation
    Contains the following substitutions: V346A, W348…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pB2HoptX

    Plasmid
    #163832
    Purpose
    Inactive two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B
    Depositor
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    MidT Substrate Y4F
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pCWXPGR-pTF-EpoRtm

    Plasmid
    #114286
    Purpose
    TET-inducible expression of Erythropoietin receptor without cytoplasmic tail
    Depositor
    Insert
    Erythropoietin Receptor (Epor Rat)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    deletion of the cytoplasmic domain
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pTU1-A-recJU

    Plasmid
    #112783
    Purpose
    Upstream homology arm, RecJ is a ssDNA specifiC exonuclease, involved in genome repair and maintenance
    Depositor
    Insert
    recJU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1221 - 1240 of 9250 results