We narrowed to 6,417 results for: nls

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hGFAP-NLS-HA-Dre-P2A-BFP

Plasmid

#51277

Purpose

Co-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoter

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS-HA-Dre-P2A-BFP

Expression

Mammalian

Promoter

hGFAP

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

Plasmid

#199252

Purpose

Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

High specificity eSpCas9(1.1)-D10A nickase

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A K848A K1003A R1060A

Promoter

CAG promoter

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-sTomato-NLS-blast

Plasmid

#136259

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

sTomato-NLS

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pUBC-SV40NLS-HoTag3-Shunzhi12nm-TSapphire

Plasmid

#250617

Purpose

Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 12nm nanoGEMs under the UBC promoter

Depositor

Liam Holt

Insert

12nm Shunzhi 60mers

Use

Lentiviral

Tags

4X(GGGGS) linker, HOTag3, SV40NLS, and TSapphire

Expression

Bacterial and Mammalian

Promoter

pUBC

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCDH-NLS-copGFP-EF1-BlastiS

Plasmid

#132772

Purpose

Spills into the cytoplasm during nuclear envelope rupture.

Depositor

Jan Lammerding

Article

Denais et al Science. 2016 Apr 15;352(6283):353-8. do

Insert

NLS-copGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mNeonGreen-NLS-blast

Plasmid

#136258

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mNeonGreen-NLS

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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BC1177-pHR-SFFV-GFP(1-10)-NLS

Plasmid

#164039

Purpose

Expression of GFP1-10 in mammalian cells

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

GFP(1-10)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-PCP-3XNeon-nls

Plasmid

#75386

Purpose

PCP-3XNeon-nls

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

PCP

Use

Lentiviral

Tags

3XmNeonGreen

Expression

Mammalian

Promoter

EFS

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin G13R

Plasmid

#60615

Purpose

Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

G13R

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

Plasmid

#86827

Purpose

Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

FLAG-NLS-MS2-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only
pLV 4xSTAR-mScarletI-NLS-blast

Plasmid

#136260

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mScarletI-NLS

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pDonor-tBFP-NLS-Neo (Universal)

Plasmid

#80767

Purpose

Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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phage ubc nls ha 2xmcp HALO

Plasmid

#64540

Purpose

Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

MCP

Use

Lentiviral

Tags

HA, HALO, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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3XFLAG-VP64-SadCas9-NLS-VP64

Plasmid

#135338

Purpose

3xFLAG 2xVP64 SadCas9 for single guide cloning

Depositor

Ronald Cohn

Article

Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

Insert

SadCas9

Tags

3xFLAG-VP64-SV40 NLS and NLS-VP64

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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phage-ubc-nls-ha-tdPCP-gfp

Plasmid

#40650

Depositor

Robert Singer

Article

Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

Insert

tdPCP-gfp

Use

Lentiviral

Tags

EGFP, HA, and NLS

Expression

Mammalian

Promoter

human ubiquitin C (UBC)

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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His-CL7-2NLS-SpyCas9(dS)-A22p3star

Plasmid

#222970

Purpose

Bacterial expression of A22p-fused SpyCas9 construct

Depositor

Jennifer Doudna

Article

Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

Insert

His-CL7-2NLS-SpyCas9(dS)-A22p3

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Promoter

tac

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

Plasmid

#100941

Purpose

Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Human, Synthetic)

Tags

1xHA and 3xTy1

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin S14C

Plasmid

#60614

Purpose

Encodes YFP and NLS tagged beta-actin with the S14C polymerization mutation

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

S14C

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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PET-HiFi SpCas9-NLS-6xHis

Plasmid

#207376

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-ArgiNLS-mKate2

Plasmid

#220607

Purpose

Flp-dependent expression of a single-cell discriminating version of mKate2 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mKate2

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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