We narrowed to 6,177 results for: nls

1. pRM053 His-TEV-Cas9 triNLS 0C

   Plasmid

   #196244

   Purpose

   Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

   Depositor

   Ross Wilson

   Article

   Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA, His6 (N terminal on backbone), NLS, Nucleopla…

   Expression

   Bacterial

   Mutation

   Cys at positions 80 and 574 have been mutated to …

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)

   Plasmid

   #133801

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA4
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and T7

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)

   Plasmid

   #134341

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIC3
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTAL-MajSat-FLAG-NLS-VP64

   Plasmid

   #69074

   Purpose

   Expresses TALE for mouse major satellite with VP64 and FLAG under CAG promoter

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

   Insert

   TALE
   

   Expression

   Mammalian

   Available Since

   Sept. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)

   Plasmid

   #133802

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA5
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and T7

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPyCAG-mKate2-3xNLS-IRES-Pac

   Plasmid

   #183618

   Purpose

   Mammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Elife. 2013 Dec 17;2:e01197. doi: 10.755

   Insert

   mKate2-3xNLS
   

   Tags

   3xNLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV 4xSTAR-mScarletI-NLS-blast

   Plasmid

   #136260

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 4 repeats
   

   Use

   Lentiviral

   Tags

   mScarletI-NLS

   Expression

   Mammalian

   Available Since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTAL-MajSat-FLAG-NLS-MCS

   Plasmid

   #69073

   Purpose

   Expresses TALE for mouse major satellite with FLAG tag under CAG promoter

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

   Insert

   TALE
   

   Expression

   Mammalian

   Available Since

   Sept. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDonor-tBFP-NLS-Neo (Universal)

   Plasmid

   #80767

   Purpose

   Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Insert

   PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRM049 His-TEV-Cas9 triNLS 2C

    Plasmid

    #196245

    Purpose

    Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-myc NES-mCE NLS

    Plasmid

    #82466

    Purpose

    Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    NES mRNA capping enzyme (Rngtt Mouse, HIV)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-EFS-PCP-3XNeon-nls

    Plasmid

    #75386

    Purpose

    PCP-3XNeon-nls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    PCP
    

    Use

    Lentiviral

    Tags

    3XmNeonGreen

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMVp-dCas9-3xNLS-VP64 (Construct 1)

    Plasmid

    #55195

    Purpose

    Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VP64

    Expression

    Mammalian

    Promoter

    CMV/hUBC

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCVL SFFV-EF1s HA.NLS.Sce(opt)

    Plasmid

    #31479

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV/Ef1s HA NLS I-SceI
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Mutation

    "opt" indicates the coding sequence has…

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEM-NLS-Cre-2A-mCherry-FKF

    Plasmid

    #119868

    Purpose

    Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Tags

    mCherry

    Expression

    Bacterial

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)

    Plasmid

    #134337

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA16_Efa
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. BC1177-pHR-SFFV-GFP(1-10)-NLS

    Plasmid

    #164039

    Purpose

    Expression of GFP1-10 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    GFP(1-10)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)

    Plasmid

    #115136

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS

    Depositor

    Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA1 anti-CRISPR protein
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. iC10 PB-zim3-mycNLS-mApple-hygro

    Plasmid

    #204719

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. hSyn Marina-T2A-nls-mCherry

    Plasmid

    #85843

    Purpose

    genetically-encoded fluorescent voltage indicator

    Depositor

    Vincent Pieribone

    Article

    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

    Insert

    GEVI Marina
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    hSyn1

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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