We narrowed to 1,403 results for: puc57

1. pDB-hPER2-mClover3-CD4-bla

   Plasmid

   #179448

   Purpose

   Donor vector to knock in mClover3 N-terminal to human PER2 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   mClover3
   

   Use

   CRISPR

   Tags

   His/Flag

   Expression

   Mutation

   Promoter

   None

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AICSDP-23:TJP1-mEGFP

   Plasmid

   #87429

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   March 21, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. AICSDP-34: TNNI1-mEGFP

   Plasmid

   #114411

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

   Insert

   TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDB-hPER2-Luciferase-hvTK-bla

   Plasmid

   #179450

   Purpose

   Donor vector to knock in firefly Luciferase C-terminal to human PER2 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   Luciferase
   

   Use

   CRISPR

   Tags

   His/Flag

   Expression

   Mutation

   Promoter

   None

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDB-hCRY1-mScarlet-I-CD4-bla

   Plasmid

   #179442

   Purpose

   Donor vector to knock in mScarlet-I C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   mScarletI
   

   Use

   CRISPR

   Tags

   His/Flag

   Expression

   Mutation

   Promoter

   None

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDB-hPER2-mScarlet-I-CD4-bla

   Plasmid

   #179449

   Purpose

   Donor vector to knock in mScarlet-I C-terminal to human PER2 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   mScarletI
   

   Use

   CRISPR

   Tags

   His/Flag

   Expression

   Mutation

   Promoter

   None

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDB-hCRY1-mClover3-CD4-bla

   Plasmid

   #179441

   Purpose

   Donor vector to knock in mClover3 C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   mClover3
   

   Use

   CRISPR

   Tags

   His/Flag

   Expression

   Mutation

   Promoter

   None

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Bxb1Donor_WT_ADRB2_LucReporter

   Plasmid

   #129788

   Purpose

   encodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

   Depositor

   Sriram Kosuri

   Article

   Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

   Insert

   ADRB2 (ADRB2 Human)
   

   Use

   Synthetic Biology

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   N/A

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDB-hCRY1-Halo-CD4-bla

   Plasmid

   #179445

   Purpose

   Donor vector to knock in Halotag C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   HaloTag (Halo7 Synthetic)
   

   Use

   CRISPR

   Tags

   His/Flag

   Expression

   Mutation

   Promoter

   None

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDB-hPER2-Halo-CD4-bla

    Plasmid

    #179451

    Purpose

    Donor vector to knock in Halotag C-terminal to human PER2 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    HaloTag (Halo7 Synthetic)
    

    Use

    CRISPR

    Tags

    His/Flag

    Expression

    Mutation

    Promoter

    None

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDB-hCRY1-Luciferase-hvTK-bla

    Plasmid

    #179444

    Purpose

    Donor vector to knock in firefly Luciferase C-terminal to human CRY1 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    Luciferase
    

    Use

    CRISPR

    Tags

    His/Flag

    Expression

    Mutation

    Promoter

    None

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDB-hCRY1-SNAP-CD4-bla

    Plasmid

    #179447

    Purpose

    Donor vector to knock in SNAPtag C-terminal to human CRY1 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    SnapTag
    

    Use

    CRISPR

    Tags

    His/Flag

    Expression

    Mutation

    Promoter

    None

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Bxb1Donor_G102H_ADRB2_LucReporter

    Plasmid

    #129794

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    G102H

    Promoter

    N/A

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFOXA2-T2A-mTagBFP2-PGK-Neo

    Plasmid

    #223191

    Purpose

    BFP reporter plasmid targeting the human FOXA2 gene

    Depositor

    Matthew Birket

    Article

    Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

    Insert

    FOXA2-T2A-mTagBFP2-PGK-Neo-FOXA2 (FOXA2 Human)
    

    Use

    Cre/Lox; Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEMS2374

    Plasmid

    #225631

    Purpose

    High Copy number plasmid containing Ple391 (DRD2 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

    Insert

    Ple391 (DRD2 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEMS2368

    Plasmid

    #225632

    Purpose

    High Copy number plasmid containing Ple385 (GPR6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

    Insert

    Ple385 (GPR6 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEMS2372

    Plasmid

    #225630

    Purpose

    High Copy number plasmid containing Ple389 (ADORA2A MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

    Insert

    Ple389 (ADORA2A Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AA320

    Plasmid

    #215952

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0 [EnAs]; sgCD47_v1 [EnAs]; DR_v1 [EnAs]; sgCD47_v2 [EnAs]}
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AA315

    Plasmid

    #215951

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control lethal

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0 [EnAs]; sgPLK1_v1 [EnAs]; DR_v1 [EnAs]; sgPLK1_v2 [EnAs]}
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AA324

    Plasmid

    #215953

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0[EnAs]; sgCD46_v4[EnAs]; DR_v1[EnAs]; sgCD47_v2[EnAs]; DR_v2[EnAs]; sgCD55_v4[EnAs];DR_v3[EnAs];sgCD81_v4[EnAs]}
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge