161,256 results

1. pcDNA3.1-GGamma10-GFP2

   Plasmid

   #166779

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma10-GFP2 (GNG10 Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHMGWA-Pa14Csy4

   Plasmid

   #41091

   Depositor

   Jennifer Doudna

   Article

   Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

   Insert

   Csy4
   

   Use

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   Wild type

   Promoter

   T7

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-CA

   Plasmid

   #69616

   Purpose

   To express gene strongly with AAV

   Depositor

   Naoshige Uchida

   Article

   Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

   Type

   Empty backbone

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_LacZ

   Plasmid

   #25893

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   LacZ
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-miniDq-P2A-mCherry

   Plasmid

   #204357

   Purpose

   AAV vector for miniDq expression under the control of human synapsin promoter

   Depositor

   Masao Doi

   Article

   Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

   Insert

   miniDq-P2A-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   The third intracellular loop (ICL3) of hM3Dq was …

   Promoter

   hSyn

   Available since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHMGWA-Pa14Csy4H29A

   Plasmid

   #41092

   Depositor

   Jennifer Doudna

   Article

   Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

   Insert

   Csy4
   

   Use

   Tags

   His6-MBP

   Expression

   Mutation

   His29Ala mmutation to abolish ribonuclease activi…

   Promoter

   T7

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pLenti-puro-ARID1A

   Plasmid

   #39478

   Purpose

   Tetracycline-inducible lentiviral expression of human ARID1A; 3rd generation lentiviral vector

   Depositor

   Ie-Ming Shih

   Article

   Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

   Insert

   ARID1A (ARID1A Human)
   

   Use

   Lentiviral

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYTK001

   Plasmid

   #65108

   Purpose

   Part Plasmid Entry Vector to be used in the Dueber YTK system.

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Insert

   none
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX458 eSp(1.1) V3

   Plasmid

   #226963

   Purpose

   CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWSK29

    Plasmid

    #172972

    Purpose

    Low copy number cloning vector

    Depositor

    Renato Morona

    Article

    Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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