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GFP-SBP-VAP-A*

Plasmid

#120175

Purpose

Expresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-A

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

VAMP associated protein A (VAPA Human)

Tags

EGFP and SBP

Expression

Mammalian

Mutation

Truncated VAP-A: 118-249

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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mCh-BICD2*-strep

Plasmid

#120168

Purpose

Expresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

BICD cargo adaptor 2 (Bicd2 Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated BICD2: 15-595 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLex 983-Micu2

Plasmid

#50372

Purpose

Expression in mammalian cells

Depositor

Vamsi Mootha

Article

Plovanich et al PLoS One. 2013;8(2):e55785. doi: 10.1371

Insert

MICU2 (MICU2 Human)

Use

Lentivirus

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-N1_MeCP2(deltaNIC)

Plasmid

#110191

Purpose

Expression of mouse MeCP2 (deltaNIC) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

delta N and C termini. Intervening region replace…

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-HA-CAD S1859A

Plasmid

#46239

Purpose

FLAG-HA-CAD with S1859 phosphorylation site mutated to alanine

Depositor

Brendan Manning

Article

Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

Insert

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Serine 1859 changed to Alanine (S1859A)

Promoter

cmv

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only
Str-STIM1-NN_neomycin

Plasmid

#65311

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

STIM1 mutated for binding motif to microtubule (STIM1 Human)

Tags

streptavidin

Expression

Mammalian

Mutation

2 amino acids of STIM1 (IP from SxIP motif) were …

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pGLOW31

Plasmid

#172698

Purpose

C. elegans mScarlet co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-3::mScarlet

Tags

mScarlet

Expression

Worm

Promoter

myo-3

Available Since

Aug. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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mCh-KIF5A*-strep

Plasmid

#120163

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5A (Kif5a Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5A: 1-559 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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TRIM28 gRNA (BRDN0001145369)

Plasmid

#76141

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM28

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM28 (TRIM28 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pET-20b-M-Ntx-S1

Plasmid

#58716

Purpose

Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

Depositor

Fiorella Tonello

Article

Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

Insert

Notexin

Tags

Methionine

Expression

Bacterial

Mutation

Changed Asparagine 1 to Serine

Promoter

T7

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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