We narrowed to 8,624 results for: FIE
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Plasmid#52271PurposeBacterial expression of human TDG-K330A C-terminally fused to GyrA intein, SUMO-E2 and GSTDepositorInserthTDG (TDG Human)
TagsGyrA-intein-Ubc9-GSTExpressionBacterialMutationK330A, SUMO acceptor site mutationPromoterT7Available SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC-mTDG-IntE2
Plasmid#52268PurposeBacterial expression of mouse TDG C-terminally fused to GyrA intein, SUMO-E2 and GSTDepositorAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJH124
Plasmid#48259PurposeTemplate plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.DepositorInsertsURA3 3' fragment
URA3 5' fragment
Ag TEF1 promoter
UseRoutine cloning vectorMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterAg TEF1 promoterAvailable SinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti_DualsgRNA_TIMM23_Puro_T2A_BFP2
Plasmid#236731PurposeDual sgRNA targeting TIMM23 expressing BFP with Puromycin selectionDepositorInsertTIMM23 (TIMM23 Human)
UseLentiviralAvailable SinceDec. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti_DualsgRNA_TIMM23_ER-dAPEX-BFP2
Plasmid#236734PurposeDual sgRNA targeting TIMM23 expressing dAPEX-BFP targeting to ERDepositorInsertTIMM23 (TIMM23 Human)
UseCRISPR and LentiviralAvailable SinceDec. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PhoCl AND A-SpyTag
Plasmid#246950PurposeExpresses multifunctional construct for AND-gated release of mCherry from materials in response to 405 nm light AND sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.DepositorInsertPlease see depositor comments below
Tags6x His tag, SsrAExpressionBacterialAvailable SinceNov. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD_LcgcC-GFP-H6
Plasmid#245528PurposeHis-tagged superfolder GFP C-terminally fused to C-terminal LCGC14 split intein fragmentDepositorInsertC-terminal Fragment of the LCGC14 PolB2 Split Intein
TagsHexahistidine Tag and Superfolder Green Fluoresce…ExpressionBacterialAvailable SinceNov. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBK1838
Plasmid#239792PurposepAAV-CMV-SNCAintron1-AlphaSyn[A53T]-WPRE-SV40pA (PD-INDUCER)DepositorInsertCMV/SNCAintron1-AlphaSyn[A53T]-WPRE-SV40pA
UseAAVAvailable SinceNov. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBK1575
Plasmid#239793PurposepLV-U6-[empty]-EFSNC-dSaCas9-[empty linker]-P2A-Puro-WPREDepositorInsertpLV-U6-[empty]-EFSNC-dSaCas9-[empty linker]-P2A-Puro-WPRE
UseLentiviralAvailable SinceNov. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNRCreCNAT
Plasmid#234430PurposePicochlorum celeri CloNat insert with nitrate-inducible Cre recombinase and loxP sites for marker and Cre removalDepositorInsertCre recombinase
UseCRISPR, Cre/Lox, and Synthetic Biology ; Green al…MutationPicochlorum celeri endoglucanase intron insertionPromoterP celeri nitrate reductaseAvailable SinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNRCreBle
Plasmid#234431PurposePicochlorum celeri ShBle insert with nitrate-inducible Cre recombinase and loxP sites for marker and Cre removalDepositorInsertCre recombinase
UseCRISPR, Cre/Lox, and Synthetic Biology ; Green al…MutationPicochlorum celeri endoglucanase intron insertionPromoterP celeri nitrate reductaseAvailable SinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
SAGA046
Plasmid#240795PurposePlasmid contains the triple selection cassette gfp -pBR322ROP tetApBR322ROP - CmTrunc that allows for in vivo recombineering in E.coli.DepositorInsertSAGA pBR322ROP His medium : T7- His tags medium- gfp -pBR322ROP tetApBR322ROP - CmTrunc
UseSynthetic BiologyMutationMutation on translation initiation region of tetAAvailable SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
SAGA045
Plasmid#240794PurposePlasmid contains the triple selection cassette gfp -pBR322ROP tetApBR322ROP - CmTrunc that allows for in vivo recombineering in E.coli.DepositorInsertSAGA pBR322ROP His low : T7- His tags low - gfp -pBR322ROP tetApBR322ROP - CmTrunc
UseSynthetic BiologyMutationMutation on translation initiation region of tetAAvailable SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
SAGA043
Plasmid#240792PurposePlasmid contains the triple selection cassette gfp -p15a tetAp15a - CmTrunc that allows for in vivo recombineering in E.coli.DepositorInsertSAGA p15a His medium : T7- His tags medium- gfp -p15a tetAp15a - CmTrunc
UseSynthetic BiologyMutationMutation on translation initiation region of tetAAvailable SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
SAGA042
Plasmid#240791PurposePlasmid contains the triple selection cassette gfp -p15a tetAp15a - CmTrunc that allows for in vivo recombineering in E.coli.DepositorInsertSAGA p15a His low : T7- His tags low - gfp -p15a tetAp15a - CmTrunc
UseSynthetic BiologyMutationMutation on translation initiation region of tetAAvailable SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
SAGA040
Plasmid#240789PurposePlasmid contains the triple selection cassette gfp -RK2 tetARK2 - CmTrunc that allows for in vivo recombineering in E.coli.DepositorInsertSAGA RK2 His medium : T7- His tags medium- gfp -RK2 tetARK2 - CmTrunc
UseSynthetic BiologyMutationMutation on translation initiation region of tetAAvailable SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
SAGA039
Plasmid#240788PurposePlasmid contains the triple selection cassette gfp -RK2 tetARK2 - CmTrunc that allows for in vivo recombineering in E.coli.DepositorInsertSAGA RK2 His low : T7- His tags low - gfp -RK2 tetARK2 - CmTrunc
UseSynthetic BiologyMutationMutation on translation initiation region of tetAAvailable SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmEos3.1-N1 GBP
Plasmid#220492PurposeExpression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos3.1 to track GFP proteins of interest to perform Fluorescent intrabody Localization MicroscopyDepositorInsertGFP Binding Protein tagged with mEos3.1
TagsmEos3.1ExpressionMammalianAvailable SinceAug. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMH-SpAsp1
Plasmid#238315PurposeSpAsp1 protein expression in bacteriaDepositorInsertasp1 (asp1 Fission Yeast)
TagsHis7-Tag, Strep-tag II, SUMO proteinExpressionBacterialMutationSpAsp1Available SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBN201
Plasmid#217917Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E mutationsDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E mutations
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y715E, Y717E, Y718EAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBN363
Plasmid#217918Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E and N711Y mutationsDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E and N711Y mutations
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y715E, Y717E, Y718E, N711YAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBN413
Plasmid#217919Purposeexpress E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E and N711Y mutationsDepositorInsertE. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E and N711Y mutations
Tagshexa-histidine tagExpressionBacterialMutationY715E, Y717E, Y718E, N711YAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GG
Plasmid#213039PurposeAAV backbone for Golden Gate assembly of donor templates using BbsI/BpiI (overhangs AGCG and GCAA, respectively)DepositorTypeEmpty backboneUseAAV; Vector for golden gate assemblyTagsN/APromoterN/AAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOPINF HopF2
Plasmid#228397PurposeRecombinant expression of His6-tagged Pseudomonas syringae pv tomato type III effector HopF2 in E. coliDepositorInsertHopF2 (hopF2 Pseudomonas syringae pv. tomato)
TagsHis6ExpressionBacterialPromoterT7 promoterAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
V5-14-3-3-e
Plasmid#229426PurposeLentiviral or overpexression of cDNADepositorAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
HA-MOB1B
Plasmid#229428PurposeLentiviral or overpexression of cDNADepositorAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest-pol2-Smarcd1-Myc
Plasmid#227716PurposeExpresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter. Has a Blasticidin resistance gene for stable cell line generation and Ampicilin for plasmid propagation.DepositorAvailable SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Vibrio_vulnificus
Plasmid#224405PurposeFor expression of the type I CBASS system encoded by Vibrio vulnificusDepositorInsertEndonuclease_Phospholipase_VvCdnG_AGS-C
ExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Vibrio_campbellii
Plasmid#224404PurposeFor expression of the type I CBASS system encoded by Vibrio campbelliiDepositorInsertEndonuclease_VcCdnG_AGS-C
ExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Pseudoalteromonas_sp._A2
Plasmid#224401PurposeFor expression of the type I CBASS system encoded by Pseudoalteromonas sp. A2DepositorInsertPsp.CdnB_AGS-C_Cap15
ExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Pectobacterium_parmentieri
Plasmid#224400PurposeFor expression of the type I CBASS system encoded by Pectobacterium parmentieriDepositorInsertPpCdnB_AGS-C_Cap15
ExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Alteromonas_sp._ALT199
Plasmid#224399PurposeFor expression of the type I CBASS system encoded by Alteromonas sp. ALT199DepositorInsertAsp.CdnB_AGS-C_Cap15
ExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Rhodanobacter_denitrificans
Plasmid#224391PurposeFor expression of the type I CBASS system encoded by Rhodanobacter denitrificansDepositorInsertPhospholipase_RdCdnA
ExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-His
Plasmid#196669PurposeExpression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
TagsTEV cleavable His10ExpressionBacterialPromoteraraBADAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
TR2
Plasmid#218403PurposeExpresses TR2 peptide in AAV9 capsidDepositorInsertTR2 7mer (RTTASLM)
UseAAVAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
NL1
Plasmid#218404PurposeNL1 insert in AAV9 capsidDepositorInsertNL1 7mer (LTTEGRR)
UseAAVAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1
Plasmid#218405PurposeTH1 insert in AAV9 capsidDepositorInsertTH1 7mer (HPARALP)
UseAAVAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
TP1
Plasmid#218406PurposeTP1 insert in AAV9 capsidDepositorInsertTP1 7mer peptide (PKYPLLG)
UseAAVAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ExSYTE.H64A
Plasmid#216262Purposeinactive ExSYTE.H64A (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoterDepositorInsertExSYTE P2A EGFP - Rat DTE
UseAAVMutationH64A mutation in each oTetRAvailable SinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTcI38-PRM-wasabi
Plasmid#195554PurposeTemperature-dependent expression of mWasabi reporter with transcription activation by TcI38DepositorInsertsmWasabi
TcI38
Tags6x His and AANDENYADAS degradation tagExpressionBacterialMutationA67T, M1V, L65S, K68R, F115L, D126G, D188GAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only