We narrowed to 8,585 results for: App
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Plasmid#45494PurposeRed intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2DepositorInsertR-GECO1.2
UseTagsExpressionMammalianMutationR-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…PromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Talin head-SpyCatcher003
Plasmid#133566PurposeExpresses EGFP-Talin head-SpyCatcher003 in mammalian cellsDepositorInsertEGFP-Talin head-SpyCatcher003
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCasKP-hph
Plasmid#117232PurposeBacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella PneumoniaeDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJM253 (miniCTX1-rhaSR-PrhaBAD)
Plasmid#110571PurposeIntegration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoterDepositorInsertrhaSR-PrhaBAD
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
plRL117
Plasmid#163635PurposeOptimized M. smegmatis CRISPRi plasmid; L5 integrating.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
G-CaMP3
Plasmid#22692Purposea single-wavelength GCaMP2-based genetically encoded calcium indicatorDepositorInsertG-CaMP3
UseTagsExpressionMammalianMutationN363D mutation (increased fluorescence)PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMD1 anti-GFP nanobody-Tn5
Plasmid#215567PurposeExpresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tagDepositorInsertanti-GFP nanobody-Tn5 fusion
UseTags3xFlag and intein-CBDExpressionBacterialMutationPromoterT7Available sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCN-mBFP
Plasmid#63847PurposeEncodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.DepositorInsertmBFP
UseTagsExpressionBacterialMutationPromoter and RBS was swapped. His-tag was removed…PromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfpC
Plasmid#111614PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(Mut3)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmIL-6 FL
Plasmid#61286Purposedrives luciferase from Wt mouse IL-6 promoter (full length)DepositorInsertIL-6 promoter (Il6 Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterIL-6 full length promoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLPO2
Plasmid#209976PurposeContains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmidsDepositorInsertreplication origin (SH71_rep)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPO1
Plasmid#209975PurposeContains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmidsDepositorInsertreplication origin (256_rep)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMpGE014
Plasmid#108682PurposeAll-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1X-Nup88
Plasmid#87338PurposeTo express human Nup88 in mammalian cellsDepositorInsertNUP88 (NUP88 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
783-Rx-hU6: RfxCas13d gRNA and array cloning backbone
Plasmid#228360PurposehU6-driven expression of RfxCas13d gRNAs and arrays compatible. Contains AarI sites for guide cloning flanked by 5' DR36DepositorTypeEmpty backboneUseCRISPR and Lentiviral; Rfxcas13d grna expression …TagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pet-MBP
Plasmid#209184PurposeExpression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coliDepositorTypeEmpty backboneUseTagsHisX6ExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-JMJD2B
Plasmid#24181PurposeMammalian expression of human JMJD2B with HA tagDepositorInsertJMJD2B (KDM4B Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53-DsbC
Plasmid#186623PurposeE. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.DepositorTypeEmpty backboneUseTagsDsbC-6His-TEVExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
T7-RelA(S536E)
Plasmid#24156DepositorInsertRelA (RELA Human)
UseTagsT7 gp10ExpressionMammalianMutationS536EPromoterAvailable sinceApril 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
CTB
Plasmid#141166PurposeExpresses recombinant Cholera Toxin B SubunitDepositorInsertCTB
UseTagsExpressionBacterialMutationNo secretion signalPromoterT7Available sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
T7-RelA(KR)
Plasmid#23251DepositorInsertNFkB RelA (RELA Human)
UseTagsT7 gp10ExpressionMammalianMutationK218/221R, K310RPromoterAvailable sinceMarch 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET15bpATnp
Plasmid#121137PurposeThis vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.DepositorInsertProtein A-Tnp
UseTagshisExpressionBacterialMutationPromoterT7Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRA1EGFP
Plasmid#193791PurposeExpresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selectionDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterPR promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-p50(1-435)
Plasmid#44747DepositorInsertp50 (Nfkb1 Mouse)
UseTagsFLAGExpressionMammalianMutationAA 1-435PromoterAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRT-KanR-FRT pA
Plasmid#82601Purpose3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selectionDepositorInsertFRT-Kan-FRT
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1deltaTMD
Plasmid#108132PurposeRecruitable cytosolic SAC1DepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(1-521)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-ChR2-EYFP-Kv
Plasmid#89256PurposeChannelrhodopsin localized to neuronal soma via a Kv2.1 targeting motifDepositorInsertChannelrhodopsin
UseAAVTagsEYFPExpressionMammalianMutationH134RPromotersynapsinAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Roxed-Cre
Plasmid#51273PurposeComponent of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by DreDepositorInsertRoxed-Cre
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBECKP-km
Plasmid#117235PurposeA cytidine deaminase-mediated base-editing plasmid in Klebsiella PneumoniaeDepositorInsertncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTP1174
Plasmid#104162PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby
Plasmid#71761PurposemGFP and Synaptophysin-mRuby expression from Flp-expressing neuronsDepositorInsertsmGFP
Synaptophysin
UseAAVTagsPalmitoylation signal and mRubyExpressionMutationPromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEXPqcxip-hSURF4-FLAG
Plasmid#159139PurposeHu SURF4 -flag mammalian expression Gateway vector.DepositorInsertsurfeit 4 (SURF4 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMV (cytomegalovirus) enhancer and the MSV (mouse…Available sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-pheS
Plasmid#111611PurposeYeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.DepositorInsertpheS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV3Tag8li-hTXLNA-3xFLAG
Plasmid#165471PurposeHuman TXLNA mammalian expression vector with 3 C-terminal FLAG tags.DepositorInserttaxilin alpha (TXLNA Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Blasto
Plasmid#167904PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsXRN1_K
Plasmid#146784PurposeMammalian Expression of HsXRN1DepositorInsertHsXRN1 (XRN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
MXS_CMV::ZeoR-bGHpA
Plasmid#62441PurposeMXS_chaining vector with CMV::ZeoR-bGHpADepositorInsertresistance cassette against Zeocin with CMV Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NADP-Snifit-mito
Plasmid#113919PurposePlasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cellsDepositorInsertNADP-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-Talin rod-mCherry
Plasmid#133567PurposeExpresses SpyTag003-Talin rod-mCherry in mammalian cellsDepositorInsertSpyTag003-Talin rod-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS156
Plasmid#182707PurposeCRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Cm
Plasmid#92052PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionMutationPromoterpRhamBAD and ptetAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mito-SNAP
Plasmid#206480PurposeEncodes a mitochondrially-targeted SNAP tag for mammalian cell expressionDepositorInsertMitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)
UseTagsSNAPfExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_Blasto
Plasmid#167856PurposePiggyBac compatible plasmid expressing spCas9DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST-2K
Plasmid#44564Purposefor ectopic constitutive expression of two mycobacterial proteins in one vectorDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-O-GECO1
Plasmid#46025PurposeOrange intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertO-GECO1
UseTagsExpressionMammalianMutationR-GECO1 N45I/A73V/S142P/M146R/M223T/M339L/K378R/E…PromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPCK
Plasmid#133128Purposea recombinant plasmid, expressing pckA PEP-carboxykinase) geneDepositorInsertPEP-carboxykinase gene
UseTagsExpressionBacterialMutationPromoterPEP-carboxykinase promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg61-PDGFR_EF1a_eGFP
Plasmid#186310PurposePlasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg61-PDGFR
eGFP
UseTagsHis, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PI4KB
Plasmid#139310PurposeRecruitable PI4KBDepositorInsertmCherry: FKBP1A(3-108): GGSA]4GG:PI4KB (PI4KB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
MAC-tag-C
Plasmid#108077PurposeGateway compatible destination vectors containing StrepIII/HA/BioIDDepositorInsertCCDB (ccdB(letD) )
UseTagsStrepIII-HA-BirA*ExpressionBacterial and MammalianMutationPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only