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pHR SFFVp MCP-mCherry IRES h2b-diRFP

Plasmid

#102351

Purpose

Lentiral expression vector bearing the MS2 Coat Protein (MCP) fused to mCherry as well as the nuclear marker histone 2b fused to two copies of irFP. Also bears the hygromycin selection marker.

Depositor

Jared Toettcher

Article

Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

Insert

MS2 Coat Protein

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

SFFV

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-EGFR-T790M-tmpknot-epegRNA

Plasmid

#214093

Purpose

Lentiviral vector expressing epegRNA to induce EGFR T790M mutation

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

EGFR T790M epegRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b-SET

Plasmid

#8822

Depositor

Judy Lieberman

Article

Beresford et al J Biol Chem 2001 Nov 16;276(46):43285-93

Insert

SET (SET Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
L1dRSLE

Plasmid

#13266

Depositor

Vance Lemmon

Article

Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

Insert

L1CAM (L1CAM Human)

Use

Tags

Expression

Bacterial

Mutation

RSLE region removed

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
AMPK-β2-MYC

Plasmid

#40606

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-β2 (Prkab2, Prkab2 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
p13xQUAS-MCS, α-cry:mCherry

Plasmid

#180481

Purpose

Inducible gene expression vector p13xQUAS-MCS, α-cry:mCherry

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

Multiple cloning site

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-GBA-N370S-tmpknot-epegRNA

Plasmid

#214091

Purpose

Lentiviral vector expressing epegRNA to induce GBA N370S mutation

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

GBA N370S epegRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET23 P53DBD

Plasmid

#174503

Purpose

express P53 DNA binding domain (94-312) in E.coli without tag

Depositor

Stewart Loh

Article

Butler et al Biochemistry. 2003 Mar 4;42(8):2396-403.

Insert

P53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)

Use

Tags

none

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAFW-Ago1

Plasmid

#50553

Purpose

Expression of FLAG tagged Ago1 in S2 cell

Depositor

Yukihide Tomari

Article

Kawamata et al Nat Struct Mol Biol. 2009 Sep;16(9):953-

Insert

Argonaute1 (AGO1 Fly)

Use

Tags

FLAG

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pBV-luc TOP2A 1kb promoter

Plasmid

#138274

Purpose

Luciferase reporter plasmid driven by TOP2A promoter

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TOP2A promoter sequence

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTU387

Plasmid

#178981

Purpose

pACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR array

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Insert

CRISPR array from Candidatus Scalindua brodae

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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EYFP-IKB

Plasmid

#111194

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

IKB-alpha

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pJ128

Plasmid

#19962

Depositor

Jonathan Tait

Article

Jin et al J Biol Chem 2004; 279:40351-57.

Insert

annexin V-128 (ANXA5 Human)

Use

Tags

(Met)-Ala-Gly-Gly-Cys-Gly-His

Expression

Bacterial

Mutation

Deletion of the initiator Met at position 1 of wi…

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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FLAG-AMPK-β2

Plasmid

#40604

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-β2 (Prkab2, Prkab2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
MBP-GTSE1-His6

Plasmid

#69109

Purpose

For bacterial expression of recombinant human GTSE1 tagged with MBP and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

G2- and S-phase Expressed 1 (GTSE1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-C-Kbp

Plasmid

#178013

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-C-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-C-Kbp

Use

Tags

Expression

Bacterial

Mutation

Promoter

araB

Available since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-Pa-Kbp

Plasmid

#178014

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-Pa-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-Pa-Kbp

Use

Tags

Expression

Bacterial

Mutation

Promoter

araB

Available since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-Hv-Kbp

Plasmid

#178015

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-Hv-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-Hv-Kbp

Use

Tags

Expression

Bacterial

Mutation

Promoter

araB

Available since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-D-Kbp

Plasmid

#178012

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-D-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-D-Kbp

Use

Tags

Expression

Bacterial

Mutation

Promoter

araB

Available since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1-C

Plasmid

#128311

Purpose

Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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