Addgene

We narrowed to 26,688 results for: Tat

Showing: 1241 - 1260 of 26688 results

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pWSK129-stm2585_S130A

Plasmid

#174403

Purpose

Bacterial expression of sarA with its GSK3-binding SxxxSP motif mutated to SxxxAP.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Use

Tags

Expression

Bacterial

Mutation

sarA with S130A mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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sg3

Plasmid

#113968

Purpose

Single short guide RNA targeting CAATGTATCTTATCATGTC

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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sg2+sg3

Plasmid

#113970

Purpose

Double short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg2+sg3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGI3EM11

Plasmid

#135483

Purpose

Agrobacterium T-DNA with SpunH2Bpr driving hph-GFP

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunH2Bpr hph-GFP ADH1t

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2Bpr

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGI3EM29

Plasmid

#135489

Purpose

Agrobacterium T-DNA with Spun H2Bpr driving hph-mCitrine

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr hph-mCitrine

Use

Tags

mCitrine

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2A/Bpr (divergent)

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGI3EM30

Plasmid

#135490

Purpose

Agrobacterium T-DNA with Spun H2Bpr driving hph-mClover3

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr hph-mClover3

Use

Tags

mClover3

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2A/Bpr (divergent)

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGI3EM31

Plasmid

#135491

Purpose

Agrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr hph-mCerulean3

Use

Tags

mCerulean3

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2A/Bpr (divergent)

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-001428gp160-754*

Plasmid

#123252

Purpose

Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (001428) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-AD8gp160-754*

Plasmid

#123257

Purpose

Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (AD8) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-191084-gp160

Plasmid

#123234

Purpose

Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (191084 B7-19) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-DU422gp160-754*

Plasmid

#123248

Purpose

Mammalian expression plasmid for Env from the DU422 HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (DU422) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-CH115gp160-754*

Plasmid

#123281

Purpose

Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (CH115) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-CH111gp160-754*

Plasmid

#123263

Purpose

Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (AD8) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-B41gp160-754*

Plasmid

#123240

Purpose

Mammalian expression plasmid for Env from the B41 HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (B41) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-25711gp160-754*

Plasmid

#123217

Purpose

Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (25711) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pVR2-m4

Plasmid

#84723

Purpose

pVR2 but with G to U and A to C mutations following the riboswitch aptamer

Depositor

Nils Walter

Article

Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

Inserts

E. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene

Use

Tags

Expression

Bacterial

Mutation

G to U mutation at the first nucleotide after the…

Promoter

Available since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-dCys

Plasmid

#110541

Purpose

Bacterial protein of E.coli DHFR C85A/C152S mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Use

Tags

Expression

Bacterial

Mutation

C85 mutated to A85, C152 mutated to S152

Promoter

T7

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCML 313

Plasmid

#98586

Purpose

pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Insert

csrB

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLacO

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCML 314

Plasmid

#98584

Purpose

pCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Inserts

CsrA
csrB

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLacO

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pJK385

Plasmid

#71572

Purpose

Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

succinyl-CoA:acetate CoA-transferase

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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