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Showing: 1241 - 1260 of 2784 results

  1. pHR_SFFV*/Puro2a-GFP

    Plasmid
    #198222
    Purpose
    Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP
    Depositor
    Eric Davis
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Codon-optimized puromycin resistance gene, follow…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198222-plasmid-map-sequence-id-389408

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  2. PB ID1-iCreERT2

    Plasmid
    #139280
    Purpose
    Human ID1-iCreERT2 transgene piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    ID1 (ID1 Human)
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Replaced the ID1 protein coding sequence in the t…
    Promoter
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    139280-plasmid-map-sequence-id-315091

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  3. TrHKII-pGFPN3

    Plasmid
    #21921
    Depositor
    Hossein Ardehali
    Article
    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.
    Insert
    Truncated human HKII cDNA (lacking the sequence encoded by exon 1, which is the mitochondrial binding domain) in pGFP-N3 plasmid (HK2 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This is the truncated human HKII cDNA sequence (l…
    Promoter
    Available since
    Oct. 1, 2009
    Availability
    Academic Institutions and Nonprofits only

  4. pSFV-Helper1

    Plasmid
    #92073
    Purpose
    This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.
    Depositor
    Henrik Garoff
    Article
    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356
    Insert
    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
    Use
    Helper plasmid, for run-off transcription and rna…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92073-plasmid-map-sequence-id-275942

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  5. KG#205

    Plasmid
    #110881
    Purpose
    Expresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Article
    Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1
    Inserts
    rab-3 promoter
    pde-4d (isoform d) D448N cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Asp 448 to Asn
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110881-plasmid-map-sequence-id-212568

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  6. KG#84

    Plasmid
    #110878
    Purpose
    Expresses the C. elegans acy--1 P260S gain-of-function cDNA in ventral cord cholinergic motor neurons
    Depositor
    Kenneth Miller
    Article
    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1
    Inserts
    unc-17beta promoter
    acy-1 P260S gain-of-function cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed Proline to Serine at amino acid 260
    Promoter
    Available since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110878-plasmid-map-sequence-id-217951

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  7. KG#81

    Plasmid
    #110876
    Purpose
    Expresses the C. elegans acy-1 P260S gain-of-function cDNA in body wall muscle
    Depositor
    Kenneth Miller
    Article
    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1
    Inserts
    myo-3 promoter
    acy-1 P260S gain-of-function cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed Proline to Serine at amino acid 260
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110876-plasmid-map-sequence-id-212571

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  8. KG#83

    Plasmid
    #110877
    Purpose
    Expresses the C. elegans acy--1 P260S gain-of-function cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Article
    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1
    Inserts
    rab-3 promoter
    acy-1 P260S gain-of-function cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed Proline to Serine at amino acid 260
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110877-plasmid-map-sequence-id-212517

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  9. pSR13

    Plasmid
    #69155
    Purpose
    read-outloxN mCherry to GFP switch, with swsn-1 promoter, gene (partially cDNA) and UTR, for integration on on ttTi5605, Mos Chr II
    Depositor
    Sander van den Heuvel
    Article
    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10
    Inserts
    mCherry
    swsn-1 (swsn-1 Nematode)
    eGFP
    Use
    Cre/Lox; Mossci
    Tags
    Expression
    Worm
    Mutation
    codon-optimzed index 1.0, codon-optimzed index 1.…
    Promoter
    rps-27 and swsn-1
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    69155-plasmid-map-sequence-id-121941

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  10. pET21b-hHNF4aORF-1

    Plasmid
    #206959
    Purpose
    The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the NheI/NotI sites of the pET21b(+), the bacterial expression vector.
    Depositor
    Catharine Ross, Reza Zolfaghari
    Insert
    HNF4a (Hepatocyte Nuclear Factor alpha4 Variant 2) (HNF4A Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    206959-plasmid-map-sequence-id-422372

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  11. CSNK1G3

    Plasmid
    #38939
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CSNK1G3 (CSNK1G3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    R174G, G187A, E302D (from cDNA isolate)
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pICE

    Plasmid
    #46960
    Purpose
    Plasmid for cloning and constitutive or doxycycline-inducible expression of cDNAs. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Steve Jackson
    Article
    Britton et al J Cell Biol. 2013 Jul 29.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46960-plasmid-map-sequence-id-66850

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  17. pHR_SFFV*/Puro2a-PAG1-GFP

    Plasmid
    #198223
    Purpose
    Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP
    Depositor
    Eric Davis
    Insert
    PAG1 (PAG1 Human)
    Use
    Lentiviral
    Tags
    Followed by EGFP, after a flexible linker and Pre…
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198223-plasmid-map-sequence-id-389384

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  18. pPD708 DsRed-Express2-ZF21

    Plasmid
    #138795
    Purpose
    Constitutive expression of DsRed-Express2-ZF21 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF21
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138795-plasmid-map-sequence-id-320733

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  19. pPD706 DsRed-Express2-ZF13

    Plasmid
    #138793
    Purpose
    Constitutive expression of DsRed-Express2-ZF13 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF13
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138793-plasmid-map-sequence-id-320376

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  20. pPD719 DsRed-Express2-ZF173

    Plasmid
    #138811
    Purpose
    Constitutive expression of DsRed-Express2-ZF173 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF173
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    138811-plasmid-map-sequence-id-322832

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Showing: 1241 - 1260 of 2784 results