We narrowed to 2,797 results for: cdna expression plasmid
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Plasmid#15405DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1 and HAExpressionBacterialMutationinsert can be released by digestion with REs NcoI…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
QUIN
Plasmid#184267PurposeA binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.DepositorInsertCPSMV RNA1 cDNA with 4 introns
UseTagsExpressionPlantMutationS1098P, K1651RPromoterCaMV 35S with duplicated enhancerAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHW1385
Plasmid#229879PurposeNegative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTRDepositorInsertPmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
UseTagsmScarlet-I-C1ExpressionWormMutationPromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1391
Plasmid#229877PurposeNegative selection marker for extrachromosomal arrays during RMCE crosses - Psnt-1::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTRDepositorInsertPsnt-1::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
UseTagsmScarlet-I-C1ExpressionWormMutationPromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLHKII-pGFPN3
Plasmid#21920DepositorInsertFull length human HKII cDNA in pGFP-N3 plasmid (HK2 Human)
UseTagsGFPExpressionMammalianMutationThis is the full length human HKII cDNA sequence …PromoterAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
KG#203
Plasmid#110880PurposeExpresses the C. elegans pde-4 (isoform d) cDNA pan-neuronallyDepositorInsertsrab-3 promoter
pde-4d (isoform d) cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV*/Puro2a-GFP
Plasmid#198222PurposeLentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFPDepositorInsertEGFP
UseLentiviralTagsCodon-optimized puromycin resistance gene, follow…ExpressionMammalianMutationPromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB ID1-iCreERT2
Plasmid#139280PurposeHuman ID1-iCreERT2 transgene piggyBac transposonDepositorInsertID1 (ID1 Human)
UseCre/LoxTagsExpressionMammalianMutationReplaced the ID1 protein coding sequence in the t…PromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
TrHKII-pGFPN3
Plasmid#21921DepositorInsertTruncated human HKII cDNA (lacking the sequence encoded by exon 1, which is the mitochondrial binding domain) in pGFP-N3 plasmid (HK2 Human)
UseTagsGFPExpressionMammalianMutationThis is the truncated human HKII cDNA sequence (l…PromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSFV-Helper1
Plasmid#92073PurposeThis is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.DepositorInsertcDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
UseHelper plasmid, for run-off transcription and rna…TagsExpressionMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
KG#205
Plasmid#110881PurposeExpresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronallyDepositorInsertsrab-3 promoter
pde-4d (isoform d) D448N cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationchanged Asp 448 to AsnPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#84
Plasmid#110878PurposeExpresses the C. elegans acy--1 P260S gain-of-function cDNA in ventral cord cholinergic motor neuronsDepositorInsertsunc-17beta promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationChanged Proline to Serine at amino acid 260PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#81
Plasmid#110876PurposeExpresses the C. elegans acy-1 P260S gain-of-function cDNA in body wall muscleDepositorInsertsmyo-3 promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationChanged Proline to Serine at amino acid 260PromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#83
Plasmid#110877PurposeExpresses the C. elegans acy--1 P260S gain-of-function cDNA pan-neuronallyDepositorInsertsrab-3 promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationChanged Proline to Serine at amino acid 260PromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSR13
Plasmid#69155Purposeread-outloxN mCherry to GFP switch, with swsn-1 promoter, gene (partially cDNA) and UTR, for integration on on ttTi5605, Mos Chr IIDepositorInsertsUseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0, codon-optimzed index 1.…Promoterrps-27 and swsn-1Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21b-hHNF4aORF-1
Plasmid#206959PurposeThe ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the NheI/NotI sites of the pET21b(+), the bacterial expression vector.DepositorInsertHNF4a (Hepatocyte Nuclear Factor alpha4 Variant 2) (HNF4A Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
CSNK1G3
Plasmid#38939PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCSNK1G3 (CSNK1G3 Human)
UseTagsHis TevExpressionBacterialMutationR174G, G187A, E302D (from cDNA isolate)PromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMyc4ElbLuc.
Plasmid#53246Purposereporter plasmid containing 4 myc cDNA binding sitesDepositorInsertpMyc4ElbLuc (MYC Human)
UseTagsExpressionMutationTo prepare plasmids with one, two, three, and fou…PromoterAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMyc3ElbLuc
Plasmid#53245Purposereporter plasmid containing 3 myc cDNA binding sitesDepositorInsertpMyc3ElbLuc (MYC Human)
UseTagsExpressionMutationTo prepare plasmids with one, two, three, and fou…PromoterAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMyc1ElbLuc,
Plasmid#53243Purposereporter plasmid containing 1 myc cDNA binding sitesDepositorInsertpMyc1ElbLuc (MYC Human)
UseTagsExpressionMutationTo prepare plasmids with one, two, three, and fou…PromoterAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only