We narrowed to 4,666 results for: cre encoding plasmid

1. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE

   Plasmid

   #162381

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8m
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   CAG

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. HCP2

   Plasmid

   #166104

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HCP3

   Plasmid

   #166105

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ATF4 2: Mouse ATF4 (CHOP11/cATF)-bZIP

   Plasmid

   #21849

   Depositor

   David Ron

   Insert

   ATF4 (Atf4 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   N-terminal transactivation domain deleted and rep…

   Promoter

   Available since

   Sept. 25, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFETCh_ATF4

   Plasmid

   #72350

   Purpose

   Donor vector for 3' FLAG tag of human ATF4

   Depositor

   Eric Mendenhall, Richard M. Myers

   Insert

   ATF4 Homology Arms (ATF4 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcGFP-N1_CBPdIDR1

   Plasmid

   #221904

   Purpose

   Transient expression of GFP-tagged CBPdeltaIDR1 in mammalian cells

   Depositor

   Daniel Bose

   Insert

   CBPdeltaIDR1 (CREBBP Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deletion of IDR1 (aa 72-175)

   Promoter

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLVX ALIX-GFP S718/721A

   Plasmid

   #138583

   Purpose

   Expresses fluorescent ALIX S718/721A in mammalian cells

   Depositor

   Wesley Sundquist

   Insert

   ALIX (PDCD6IP Human)
   

   Use

   Lentiviral

   Tags

   GFP and h30

   Expression

   Mammalian

   Mutation

   changed serines 718 and 721 to alanines

   Promoter

   TREtight

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAcGFP-N1_CBPdIDR6

   Plasmid

   #221907

   Purpose

   Transient expression of GFP-tagged CBPdeltaIDR6 in mammalian cells

   Depositor

   Daniel Bose

   Insert

   CBPdeltaIDR6 (CREBBP Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deletion of IDR6 (aa 1856-2090)

   Promoter

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAcGFP-N1_CBPdIDR4

   Plasmid

   #221906

   Purpose

   Transient expression of GFP-tagged CBPdeltaIDR4 in mammalian cells

   Depositor

   Daniel Bose

   Insert

   CBPdeltaIDR4 (CREBBP Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deletion of IDR4 (aa 662-787)

   Promoter

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAcGFP-N1_CBPdIDR7

    Plasmid

    #221908

    Purpose

    Transient expression of GFP-tagged CBPdeltaIDR7 in mammalian cells

    Depositor

    Daniel Bose

    Insert

    CBPdeltaIDR7 (CREBBP Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deletion of IDR7 (aa 2109-2434)

    Promoter

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAcGFP-N1_CBPdIDR3

    Plasmid

    #221905

    Purpose

    Transient expression of GFP-tagged CBPdeltaIDR3 in mammalian cells

    Depositor

    Daniel Bose

    Insert

    CBPdeltaIDR3 (CREBBP Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deletion of IDR3 (aa 441-587)

    Promoter

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCLX-KRT7-GFP

    Plasmid

    #216853

    Purpose

    This plasmid is for the production of lentiviral vectors that can be used to identify and live sort pancreatic progenitor expresssing the Keratin7 gene.

    Depositor

    Patrick Salmon

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Keratin7

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Mouse 5' Srcap AID GFP Puro

    Plasmid

    #127903

    Purpose

    HDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Srcap Gene

    Depositor

    Mark Groudine

    Insert

    Srcap (Srcap Mustard Weed, Mouse)
    

    Use

    Donor plasmid

    Tags

    GFP, AID, PuroR

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV8-hSyn-flex-miR30-eGFP-shNts

    Plasmid

    #132717

    Purpose

    Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Nts gene which encodes neurotensin

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    GFP-shNts (Nts Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Synapsin 1

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-DIO-ASAP3Kv-WPRE

    Plasmid

    #132330

    Purpose

    expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3KV
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HCP1

    Plasmid

    #166103

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.

    Depositor

    Matthias Heinemann

    Insert

    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiCRISPR v2-Blast

    Plasmid

    #83480

    Purpose

    Mammalian expression of Cas9 and sgRNA scaffold

    Depositor

    Mohan Babu

    Insert

    Blasticidin S deaminase
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Replaced puromycin N-acetyltransferase on the ori…

    Promoter

    EF-1α

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSc1-puro

    Plasmid

    #80438

    Purpose

    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin

    Depositor

    Ervin Welker

    Article

    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

    Inserts

    sp Cas9 gRNA
    eGFP
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6, and hPGK

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX ALIX-GFP S718/721/E

    Plasmid

    #138584

    Purpose

    Expresses fluorescent ALIX S718/721E in mammalian cells

    Depositor

    Wesley Sundquist

    Insert

    ALIX (PDCD6IP Human)
    

    Use

    Lentiviral

    Tags

    GFP and h30

    Expression

    Mammalian

    Mutation

    changed serines 718 and 721 to glutamic acids

    Promoter

    TREtight

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAcGFP-N1_CBPdIDR7-H1

    Plasmid

    #221930

    Purpose

    Transient expression of GFP-tagged CBPdeltaIDR7-H1 in mammalian cells

    Depositor

    Daniel Bose

    Insert

    CBPdeltaIDR7-H1 (CREBBP Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deletion of IDR7, CBP aa 2187 - 2216

    Promoter

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge