We narrowed to 2,389 results for: dap

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Syntaxin-4 M2 (Y115A, Y148A, Y251A)

Plasmid

#44030

Depositor

Thomas Weimbs

Article

Reales et al PLoS One. 2011;6(6):e21181. doi: 10.1371

Insert

Syntaxin-4

Tags

2xMyc and His

Expression

Mammalian

Mutation

Y115A Y148A Y251A

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
AIRAP 5: AIRAP(1-50).GST,V1.pcDNA3

Plasmid

#21795

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

AIRAP (Zfand2a Mouse)

Tags

GST

Expression

Mammalian

Mutation

amino acids 1-50

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pCRISPRi

Plasmid

#199808

Purpose

vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

Depositor

Steve Salipante

Article

Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

Inserts

dCas9
sgRNA with GFP cassette

Use

CRISPR

Promoter

P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCnCre

Plasmid

#232907

Purpose

Electroporation vector for expression of Cre recombinase in Cuprividus necator

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

Insert

Cre recombinase (cre Synthetic)

Use

Cre/Lox and Synthetic Biology

Promoter

Ptac-cymO

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-PGK-Zeo

Plasmid

#227271

Purpose

Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-Ef1a-Zeo

Plasmid

#227268

Purpose

Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHInt-AppA-KanR-loxP

Plasmid

#232910

Purpose

Plasmid for marker free integration of E. coli appA at the phac1 locus of Cupriavidus necator.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

Insert

AppA (appA )

Use

Synthetic Biology

Promoter

Pj5

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCHC042 (∆phaCAB)

Plasmid

#226208

Purpose

Conjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for phaCAB

Use

Synthetic Biology

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-AAVS1-Ef1a-Blast

Plasmid

#227267

Purpose

Empty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-Ef1a-Puro

Plasmid

#227266

Purpose

Empty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-PGK-Puro

Plasmid

#227269

Purpose

Empty AAVS1 targeting donor for the insertion of Puro and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCMVtight-RFP-IRES-CD

Plasmid

#126678

Purpose

Doxycycline inducible expression of CD along with reporter RFP

Depositor

Joan Massague

Article

Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

Insert

RFP-IRES-CD (FCY1 Budding Yeast)

Promoter

tight TRE

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-H2BC11

Plasmid

#207755

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-PGK-Blast

Plasmid

#227270

Purpose

Empty AAVS1 targeting donor for the insertion of Blast and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE2-Bla(HA–RILP-FLAG)

Plasmid

#102424

Purpose

Dual tagged RILP for expression in mammalian cells.

Depositor

Steven Weinman

Article

Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

Rab interacting lysosomal protein (RILP Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

Tet-Responsive

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

Plasmid

#129774

Purpose

Marker of microtubule dynamics in C. elegans hypodermis

Depositor

Andrew Chisholm

Article

Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

Inserts

Pcol-19
ebp-2 (ebp-2 Nematode)
unc-54 3'UTR

Use

Synthetic Biology; Gateway

Tags

Synthetic Intron and mGFP

Expression

Worm

Promoter

Pcol-19

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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EP-Pol vector

Plasmid

#131686

Purpose

A lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymerase

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Insert

Adenovirus DNA polymerase

Use

Lentiviral

Tags

HA

Mutation

F421Y, D827A

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Barr2 flag

Plasmid

#14685

Depositor

Robert Lefkowitz

Article

Laporte et al Proc Natl Acad Sci U S A. 1999 Mar 30. 9

Insert

beta-arrestin 2 (Arrb2 Rat)

Tags

flag

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pMRX-IPU-FLAG-NEK9

Plasmid

#168269

Purpose

Expresses NEK9 in mammalian cells

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

NEK9 (NEK9 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pUASp-GBP-Tbro

Plasmid

#179995

Purpose

Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.

Depositor

Graydon Gonsalvez

Article

Baker et al Development. 2021 Nov 15;148(22). pii: 2

Insert

GBP nanobody

Tags

Trbo

Expression

Insect

Promoter

UAS-Gal4

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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