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Plasmid#234169PurposeHeterologous protein expression of 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase in Escherichia coliDepositorInsert1HKA (folK Escherichia coli)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_6BIO
Plasmid#234152PurposeHeterologous protein expression of clan CA, family C40, NlpC/P60 superfamily cysteine peptidase in Escherichia coliDepositorInsert6BIO (TVAG_298080 Trichomonas vaginalis)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_4WDC
Plasmid#234153PurposeHeterologous protein expression of DELTA-actitoxin-Afr1a (DELTA-AITX-Afr1a) (Fragaceatoxin C) (fraC) in Escherichia coliDepositorInsert4WDC
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_2H2Z
Plasmid#234147PurposeHeterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coliDepositorInsert2H2Z (ORF1ab Severe acute respiratory syndrome-related coronavirus)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZIKV isolate PRVABC59 NS3 helicase domain
Plasmid#228664PurposeBacterial expression of the Zika virus NS3 helicaseDepositorInsertZika virus NS3 helicase
UseTagsHis-GST-TEVExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
L1PB1 - pSPCH--PhiC31-tUBQ1 - pMUTE--Bxb1-tUBQ10
Plasmid#219699PurposeLevel 1 PhiC31 and Bxb1 integrase construct for tracking of stomata development: pSPCH promoter for PhiC31 and pMUTE promoter for Bxb1, no tagDepositorInsertpSPCH::PhiC31:tUBQ1 - pMUTE::Bxb1:tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)
Plasmid#219689PurposeLevel 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PsB (mtagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJ1-g1g2-K2
Plasmid#218217PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs, MoClo compatibleDepositorInsertAtU6 promoters and sgRNA scaffolds
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4
Plasmid#219711PurposeLevel 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.DepositorInsertGG0-pAHP6:PhiC31-NLS:tUBQ1-GG4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-N
Plasmid#218398Purpose3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_N-H1
Plasmid#218397Purpose3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK7-g7g8-K8
Plasmid#218220PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAsDepositorInsertAtU6 promoters and sgRNA scaffolds
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK3-g3g4-K4
Plasmid#218218PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAsDepositorInsertAtU6 promoters and sgRNA scaffolds
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK5-g5g6-K6
Plasmid#218219PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAsDepositorInsertAtU6 promoters and sgRNA scaffolds
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
Plasmid#219687PurposeLevel 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPtGG-1
Plasmid#89990PurposepBR322-based entry vector for Gateway cloningDepositorTypeEmpty backboneUseGolden gate entry vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
MERS-CoV Mpro strain HCoV-EMC 3CL protease domain
Plasmid#228646PurposeBacterial expression of the MERS-CoV Mpro domain for biochemical assay.DepositorInsertMERS-Cov Mpro domain
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_6IYE
Plasmid#234237PurposeHeterologous protein expression of peptidyl-tRNA hydrolase (PTH) in Escherichia coliDepositorInsert6IYE (pth Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_1T3Y
Plasmid#234228PurposeHeterologous protein expression of coactosin-like protein in Escherichia coliDepositorInsert1T3Y (COTL1 Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_2WWE
Plasmid#234210PurposeHeterologous protein expression of PI3K-C2-gamma in Escherichia coliDepositorInsert2WWE (PIK3C2G Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits