We narrowed to 8,680 results for: sgRNA

1. Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#1

   Plasmid

   #208387

   Purpose

   Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#1 gene.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   sgZBP1 (Zbp1 Mouse)
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#2

   Plasmid

   #208388

   Purpose

   Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#2 gene.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   sgZBP1 (Zbp1 Mouse)
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX601-miniCMV-SaCas9-U6-YFPvsSaCas9 sgRNA

   Plasmid

   #107050

   Purpose

   All-in-one vectors expressing both SaCas9 and YFP sgRNA

   Depositor

   Alex Hewitt

   Insert

   SaCas9
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124543

   Purpose

   Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
   

   Expression

   Mammalian

   Mutation

   d11-41 C55G, d11-41

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)

   Plasmid

   #160571

   Purpose

   Version of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   sgRNAscaffold 2.1 scRNA
   

   Use

   CRISPR

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)

   Plasmid

   #181787

   Purpose

   Expresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ShTnsB, ShTnsC, ShCas12k-ShTniQ
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1-puro U6 sgRNA Oct4A -158

   Plasmid

   #50922

   Purpose

   U6 driven sgRNA targeting OCT4 isoform A -158 bp from TSS

   Depositor

   Scot Wolfe, Rene Maehr

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   Oct4A -158 sgRNA (POU5F1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMD19T-slr0230-PL22-sgRNANT1-KmR

   Plasmid

   #73224

   Purpose

   Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli

   Depositor

   Paul Hudson

   Article

   Yao et al ACS Synth Biol. 2015 Dec 28.

   Insert

   PL22-sgRNA NT1
   

   Expression

   Bacterial

   Promoter

   PL22

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKSB-611U6-sgRNA3 AGAG AACA

   Plasmid

   #173673

   Purpose

   To clone and express a gRNA in Anopheles

   Depositor

   Eric Marois

   Article

   Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-sgRNA-Cre-GpNLuc-sgmcGAS

    Plasmid

    #208385

    Purpose

    Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine cGAS gene.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    sgcGAS (Cgas Mouse)
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-sgRNA-Cre-GpNLuc-sgmSTING

    Plasmid

    #208386

    Purpose

    Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine STING gene.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    sgSTING (Sting1 Mouse)
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 8xCTS1_WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

    Plasmid

    #124546

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro
    

    Expression

    Mammalian

    Mutation

    WT, WT

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA

    Plasmid

    #133342

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKSB-631 U6-sgRNA3/4 AACA GCTT

    Plasmid

    #173675

    Purpose

    To clone and express a gRNA in Anopheles

    Depositor

    Eric Marois

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-U6-sgRNA-PGK-puromycin-AflII

    Plasmid

    #106404

    Purpose

    Empty guide RNA cloning vector with an AflII site added from Addgene 41824

    Depositor

    Jason Gertz

    Article

    Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-Flag-eSpCas9 (without sgRNA)

    Plasmid

    #92354

    Purpose

    Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMuLE ENTR U6 stuffer sgRNA scaffold R4-R3

    Plasmid

    #62131

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    CRISPR; Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Px458-Cas9n-Trp63-Exon4-1sgRNA

    Plasmid

    #88848

    Purpose

    CRISPR KO of Trp63

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp63 (Trp63 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

19. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid

    #129531

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMuLE ENTR U6 stuffer sgRNA scaffold L1-L4

    Plasmid

    #62128

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    CRISPR; Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge