We narrowed to 45,029 results for: cha

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pLenti-VQR-P2A-GFP-PGK-Puro

Plasmid

#110861

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-BRD4ΔN-miRFP670-Cry2WT

Plasmid

#122439

Purpose

Expresses disordered protein BRD4(462-1362) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

BRD4 (BRD4 Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Mutation

Deleted amino acids 1-461

Promoter

SFFV

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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hum alpha ENAC promoter a25-a23

Plasmid

#83427

Purpose

promoter reporter construct to test 5' flanking sequences

Depositor

Christie Thomas

Article

Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

Insert

human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)

Use

Luciferase

Tags

Luciferase

Promoter

alpha ENAC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pC-SUR-YR

Plasmid

#61768

Purpose

Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).

Depositor

Ken Haynes

Article

Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

Inserts

Sur gene
2 micron origin or replication and URA3 gene for S. cerevisiae

Use

Ternary vector for agrobacterium mediated transfo…

Promoter

Magnaporthe oryzae ILV promoter

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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natMX6-ins5

Plasmid

#195042

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

NatR

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pROS11

Plasmid

#107925

Purpose

amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-S (Wuhan variant)

Plasmid

#169846

Purpose

Mammalian expression plasmid for S of SARS-CoV-2 (Wuhan variant) with N-terminal c-myc tag

Depositor

Erik Procko

Article

Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

Insert

Protein S

Tags

HA leader and c-myc tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-HA-Slc1a5 (mouse)

Plasmid

#71458

Purpose

inducible expression fo Slc1a5 in mammalian cells

Depositor

Shao-Cong Sun

Article

Nakaya et al Immunity. 2014 May 15;40(5):692-705. doi

Insert

Slc1a5 (Slc1a5 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

A85P, L125F and V384A mutations compared to GenBa…

Promoter

CMV

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only
ReactionC_pBIG1a_ATG7_ATG10

Plasmid

#190860

Purpose

Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG7 (ATG7 Human)
ATG10 (ATG10 )

Expression

Bacterial and Insect

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pαH-S-HRV3C

Plasmid

#164567

Purpose

SARS-CoV-2 Spike protein with furin site mutated to HRV3C site (S-HRV3C variant)

Depositor

Priyamvada Acharya

Article

Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

Insert

Spike - Furin site changed for HRV3C site

Tags

2X Strep-Tag II and 8X His tag

Expression

Mammalian

Mutation

682-685 (furin site) replaced with HRV3C site (LE…

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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