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EBFP2-Cx43

Plasmid

#69020

Purpose

Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx43 (Gja1 Rat)

Tags

EBFP2

Promoter

CMV

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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C1-F-tractin(delta1-6)-mCitrine

Plasmid

#155219

Purpose

F-actin bidning mutant of F-tractin

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)

Tags

mCitrine

Expression

Mammalian

Mutation

Deletion of first 6 AA from F-tractin (9-14 from …

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLexm-gfp-Pept2

Plasmid

#167988

Purpose

expresses rat PepT2 with a c terminal cleavable GFP-His tag.

Depositor

Simon Newstead

Article

Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

Insert

PepT2 (Slc15a2 Rat)

Tags

cleavable GFP-his8

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pRK7-HA-S6K1-F5A-E389

Plasmid

#8988

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Mutation

F5A, T389E

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-mGold-Lysosome-N-20

Plasmid

#158006

Purpose

Visualization of lysosome in mammalian cells using mGold

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

LAMP1 (Lamp1 Rat)

Tags

mGold

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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p3E LAP2b

Plasmid

#105978

Purpose

3' element for Gateway cloning, lamina-associated polypeptide 2

Depositor

Caren Norden

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

LAP2b

Expression

Bacterial

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-ASPM CTR

Plasmid

#27669

Depositor

Joseph Loturco

Article

Paramasivam et al Cell Cycle. 2007 Jul 1. 6(13):1605-12.

Insert

Abnormal spindle-like microcephaly associated (Aspm Rat)

Tags

GFP

Expression

Mammalian

Mutation

Contains carboxy terminal region of ASPM (amino a…

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
FH95pUp95GW (B4)

Plasmid

#74013

Purpose

lentiviral expression of shRNA resistant Psd95-alpha-EGFP fusion and Psd95 shRNA

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 AU1 mTOR S2035W

Plasmid

#15678

Depositor

John Lawrence

Article

McMahon et al Mol Cell Biol. 2002 Nov . 22(21):7428-38

Insert

mTOR (Mtor Rat)

Tags

AU1

Expression

Mammalian

Mutation

S2035W

Available Since

Sept. 24, 2007

Availability

Academic Institutions and Nonprofits only
pXCX.CMV.CREB.WPRE

Plasmid

#38050

Depositor

James Uney

Article

Glover et al Neuroreport. 2004 May 19;15(7):1171-5.

Insert

CREB (Creb1 Rat)

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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p70 delta2-46 delta CT104

Plasmid

#1898

Depositor

Joseph Avruch

Article

Weng et al Mol Cell Biol 1995 May;15(5):2333-40.

Insert

p70 alpha1 S6 kinase (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Mutation

delta2-46, deltaCT104 (deletion of aa 2-46 and am…

Available Since

March 17, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-Cx43-Myc

Plasmid

#60693

Purpose

mammalian expression of Myc-tagged rat Cx43

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
Geph CR3m shRNA

Plasmid

#121069

Purpose

Negative control shRNA for plasmid 121068: shRNA targeting the coding region of the gephyrin mRNA

Depositor

Angel de Blas

Article

Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

Insert

GPHN shRNA (Gphn Rat)

Use

RNAi

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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PVALB mAb_BCN14.1.1C9_HC_Rat IgG2a

Plasmid

#182890

Purpose

Mammalian expression of PVALB rat mAb HC gene

Depositor

Heng Zhu

Insert

anti-PVALB (human) heavy chain (PVALB Rat)

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

No

Promoter

CMV

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin 219-465

Plasmid

#45972

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin 219-465 (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Dele…

Promoter

tac

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pLenLox_U6 Nlgn1

Plasmid

#59339

Purpose

Lentiviral expression vector: Neuroligin 1 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

Depositor

Peter Scheiffele

Article

Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

Inserts

Neuroligin 1 shRNA (rat) (Nlgn1 Rat)
eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG and mouse U6

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-rat PKA-RIIbeta

Plasmid

#45522

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII beta

Expression

Mammalian

Available Since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

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VAChT-SNAP

Plasmid

#105318

Purpose

VAChT tagged with SNAP

Depositor

Justin Taraska

Article

Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

Insert

VAChT (Slc18a3 Rat)

Tags

SNAP tag

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cx43-YA/VD-GFP

Plasmid

#60703

Purpose

mammalian expression of rat Cx43 YA/VD mutant fused to GFP

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

YA/VD (Y286A/V289D)

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
mApple-CathespinB-6

Plasmid

#54871

Purpose

Localization: Lysosomes, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Insert

CathespinB

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only