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pET-28b His6-TEV-SHP2-FL(D106P)

Plasmid

#245203

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

D106P

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(I282V)

Plasmid

#245210

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

I282V

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(A72V)

Plasmid

#245200

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

A72V

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(T356L)

Plasmid

#245211

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

T356L

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(P424L)

Plasmid

#245212

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

P424L

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(D61Y)

Plasmid

#245198

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

D61Y

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(H114W)

Plasmid

#245205

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

H114W

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(E69K)

Plasmid

#245199

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

E69K

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(L43F)

Plasmid

#245224

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

L43F

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pWJ 1781 URA c-Src KD

Plasmid

#245256

Purpose

Expression of the kinase domain of c-Src in yeast

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

Kinase domain of c-Src

Expression

Yeast

Mutation

wild-type

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pWJ 1781 URA v-Src FL

Plasmid

#245255

Purpose

Expression of full-length viral Src in yeast

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

Full-length viral Src

Expression

Yeast

Mutation

wild-type

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pEM1_Euk_2NLS-WT ( LbCas12a)-2NLS

Plasmid

#244836

Purpose

Mammalian expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

LbCas12a

Use

CRISPR

Tags

2xSV40 NLS

Expression

Mammalian

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEM4_CL7_Bac_2NLS-WT (LbCas12a)-4NLS

Plasmid

#244839

Purpose

Bacterial expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

LbCas12a

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEM8_2CTH10_Bac_Lbm6 (LbCas12a)-4NLS

Plasmid

#244843

Purpose

Bacterial expression of Lbm6-LbCas12a with 10xHis-MBP at N-temrinus and 4xSV40 NLS at C-temrinus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Lbm6-Cas12a

Use

CRISPR

Tags

10xHis-MBP and 4xSV40 NLS

Expression

Bacterial

Mutation

D535G/S551F/D665N

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA_H1-5

Plasmid

#223820

Purpose

Bacterial expression of SynPspA H1-5 with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Mutation

truncation of helix 0

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA

Plasmid

#223818

Purpose

Bacterial expression of SynPspA with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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BtA1N

Plasmid

#238979

Purpose

In vitro transcription of bovine arrestin-1

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al J Biol Chem. 2004 Jan 9;279(2):1262-8. d

Insert

arrestin-1 (SAG Bovine)

Use

In vitro transcription

Mutation

silent

Promoter

SP6

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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BtA2N

Plasmid

#238980

Purpose

In vitro transcription of bovine arrestin-2 long isoform

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al J Biol Chem. 2004 Jan 9;279(2):1262-8. d

Insert

arrestin-2 long (ARRB2 Bovine)

Use

In vitro transcription

Mutation

silent

Promoter

SP6

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINS-HOIP NZF1 (351-379)

Plasmid

#235655

Purpose

Bacterial expression of human HOIP NZF1 (351-379)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

HOIP NZF1 (RNF31 Human)

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 351-379 only

Promoter

T7

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-ZRANB3 NZF (619-650)

Plasmid

#235654

Purpose

Bacterial expression of human ZRANB3 NZF (619-650)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

ZRANB3 NZF (ZRANB3 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 619-650 only

Promoter

T7

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-Sharpin NZF (347-376)

Plasmid

#235651

Purpose

Bacterial expression of human Sharpin NZF (347-376)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

Sharpin NZF (SHARPIN Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 347-376 only

Promoter

T7

Available Since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-flx-fDIO-HA-Rpl10a-T2A-myc-hM3D(Gq)

Plasmid

#223654

Purpose

Expresses vTRAP and hM3D(Gq) in a FlpON-CreOFF dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

vTRAP-T2A-hM3D(Gq)

Use

AAV

Tags

HA

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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SpyCatcher-P-Domain

Plasmid

#201193

Purpose

Expresses furin P-domain genetically fused to SpyCatcher001 in bacteria

Depositor

Vesa Hytönen

Article

Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

Insert

SpyCatcher-P-Domain

Tags

HisTag and SpyCatcher001

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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hSyn-tractin

Plasmid

#187649

Purpose

Expresses F-tractin (from ITPKA)-mNeongreen under control of human synapsin promoter.

Depositor

Erin O'Shea

Article

Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

Insert

F-tractin

Use

AAV

Tags

mNeonGreen

Expression

Mammalian

Promoter

Synapsin

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1559

Plasmid

#218590

Purpose

Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimer

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

GES

Tags

ePTS1

Expression

Yeast

Mutation

Erg20(F96W, N127W), Erg20-Erg20 synthetic homodim…

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-glucose

Plasmid

#225252

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucose

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-glucose

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-Nm2Cas9-BlastR

Plasmid

#220980

Purpose

Expresses human codon-optimized Nm2Cas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

Nm2Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-CjCas9-BlastR

Plasmid

#220979

Purpose

Expresses human codon-optimized CjCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

CjCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-St1Cas9-BlastR

Plasmid

#220976

Purpose

Expresses human codon-optimized St1Cas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

St1Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-SlugCas9-HF-BlastR

Plasmid

#220973

Purpose

Expresses human codon-optimized SlugCas9-HF

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SlugCas9-HF

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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