We narrowed to 9,931 results for: SEC

1. pKM50

   Plasmid

   #123656

   Purpose

   Encodes Renilla luciferase under the Aedes aegypti ubiquitin UbL40 promoter for constitutive expression in mosquito cells

   Depositor

   Kevin Maringer

   Article

   Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

   Insert

   Aedes aegypti UbL40 promoter
   

   Use

   Luciferase

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UbL40 promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST40-CES2A-ΔC-S227A

   Plasmid

   #200556

   Purpose

   Mammalian expression of secreted mouse CES2A S227A mutant

   Depositor

   Jonathan Long

   Article

   Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

   Insert

   Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
   

   Use

   Tags

   6xHis, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Tbg-CES2A-ΔC

   Plasmid

   #200558

   Purpose

   Secreted mouse CES2A driven by heptaocyte-specific promoter in AAV viral vector

   Depositor

   Jonathan Long

   Article

   Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

   Insert

   Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mutation

   Promoter

   TBG promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUb-Cas9-mCherry_cU6:6-sgRNA

   Plasmid

   #190598

   Purpose

   The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter

   Depositor

   Claudia Rueckert

   Article

   Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR

   Tags

   mCherry - separated by T2A

   Expression

   Mutation

   Promoter

   Aedes aegypti polyubiquitin and Culex quinquefasc…

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST40-CES2C-ΔC-S230A

   Plasmid

   #200557

   Purpose

   Mammalian expression of secreted mouse CES2C S230A mutant

   Depositor

   Jonathan Long

   Article

   Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

   Insert

   Mouse Carboxylesterase 2C S230A mutant delta C-terminal (Ces2c Mouse)
   

   Use

   Tags

   6xHis, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIDS-LSD1-C

   Plasmid

   #109160

   Purpose

   Encodes a human C-terminal LSD1 fragment to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Inui et al Protein Sci. 2017 May;26(5):997-1011. do

   Insert

   human C-terminal LSD1, sequence-optimized for insect cells (KDM1A Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   p10

   Available since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-pHluorin_M153R-CD63-mScarlet

   Plasmid

   #172118

   Purpose

   Dual-color fluorescent reporter for CD63-positive exosome secretion and uptake

   Depositor

   Alissa Weaver

   Article

   Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

   Insert

   CD63 (CD63 Human)
   

   Use

   Lentiviral

   Tags

   mScarlet and pHluorin_M153R in a small extracellu…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-pHluorin_M153R-CD63

   Plasmid

   #172117

   Purpose

   Fluorescent reporter for CD63-positive exosome secretion

   Depositor

   Alissa Weaver

   Article

   Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

   Insert

   CD63 (CD63 Human)
   

   Use

   Lentiviral

   Tags

   pHluorin_M153R in a small extracelluar loop of CD…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. NES-EGFP-PH-ARNO2G-I303Ex2

   Plasmid

   #116868

   Purpose

   PIP3 biosensor

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   CYTH2 (CYTH2 Human)
   

   Use

   Tags

   EGFP and X.leavis map2k1.L(32-44)

   Expression

   Mammalian

   Mutation

   amind acids 252-399, isoleucine 303 changed to gl…

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Proinsulin-NanoLuc in pLX304

    Plasmid

    #62057

    Purpose

    Luminescent reporter of insulin secretion

    Depositor

    David Altshuler

    Article

    Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

    Insert

    Proinsulin-NanoLuc (Ins2 Synthetic, Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Inserted NanoLuc into C-peptide of mouse Ins2 cod…

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastbac1-Flag-FANCD2

    Plasmid

    #134904

    Purpose

    codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    FANCD2 (FANCD2 Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    fully synthetic, codon optimized for insect expre…

    Promoter

    polyhedron

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastbac-Dual-StrepII-BARD1

    Plasmid

    #137166

    Purpose

    Expresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell system

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    BARD1 (BARD1 Human)
    

    Use

    Tags

    StrepII

    Expression

    Insect

    Mutation

    fully synthetic, codon optimized for insect expre…

    Promoter

    p10

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-αIFNα-ab

    Plasmid

    #110799

    Purpose

    Expression and secretion of an scFv antibody (clone) 4EA1 against all mouse interferon α subtypes in mammalian cells

    Depositor

    Thomas Böldicke

    Article

    Bussow et al PLoS One. 2019 Apr 16;14(4):e0215062. do

    Insert

    antibody against mouse interferon alpha
    

    Use

    Tags

    ER signal peptide, His6 tag, and myc epitope tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-Tbg-CES2A-ΔC-S227A

    Plasmid

    #200560

    Purpose

    Secreted mouse CES2A S227A mutant driven by heptaocyte-specific promoter in AAV viral vector

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mutation

    Promoter

    CMV promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-Tbg-CES2C-ΔC

    Plasmid

    #200559

    Purpose

    Secreted mouse CES2C driven by heptaocyte-specific promoter in AAV viral vector

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2C delta C-terminal (Ces2c Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mutation

    Promoter

    TBG promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

    Plasmid

    #169166

    Purpose

    Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

    Insert

    3xFlag-6His-nsp15 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    3xFlag-6His

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCpep-E1GFP

    Plasmid

    #233884

    Purpose

    Expression of a pH sensitive variant of EGFP inserted in the C-peptide of insulin for pH measurement in insulin secretory granules

    Depositor

    Francesco Cardarelli

    Article

    De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

    Insert

    Proinsulin-E1GFP (Ins2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Inserted E1GFP into C-peptide of mouse Ins2 codin…

    Promoter

    CMVF

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-Tbg-CES2C-ΔC-S230A

    Plasmid

    #200561

    Purpose

    Secreted mouse CES2C S230A mutant driven by heptaocyte-specific promoter in AAV viral vector

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2C S230A mutant delta C-terminal (Ces2c Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mutation

    Promoter

    TBG promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBIG1b_nsp7 (SARS-CoV-2)

    Plasmid

    #169178

    Purpose

    Baculoviral transfer vector to express untagged nsp7 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp7 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    untagged

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBIG1c_nsp8 (SARS-CoV-2)

    Plasmid

    #169179

    Purpose

    Baculoviral transfer vector to express untagged nsp8 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    untagged

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge