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iTol2Amp-γ-crystallin:RFP

Plasmid

#108455

Purpose

This plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

Insert

Tol2-Amp-y-cryst-mCherry

Use

Gateway

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19/ER-mChe

Plasmid

#183163

Purpose

Construct for transient expression of endoplasmic reticulum-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

ER-mCherry

Tags

25 aa ER signal of Glycine soja kunitz-type tryps…

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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amilCP chromoprotein promoter-less

Plasmid

#118008

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, amilCP

Use

Synthetic Biology; Escherichia coli

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pre_IV_CS10_1545_v1

Plasmid

#126861

Purpose

4x 1545 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

IV_CS10_1545_v1

Use

Synthetic Biology

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_SypHer3s_cyto

Plasmid

#238971

Purpose

Mammalian neurons SypHer3s expression

Depositor

Dmitry Bilan

Article

Lanin et al J Biophotonics. 2020 Mar;13(3):e20190024

Insert

SypHer3s

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_GFAP_SypHer3s_cyto

Plasmid

#238969

Purpose

Mammalian astrocytes SypHer3s expression

Depositor

Dmitry Bilan

Article

Lanin et al J Biophotonics. 2020 Mar;13(3):e20190024

Insert

SypHer3s

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-hAAT-W

Plasmid

#126704

Purpose

Constituitive, ubiquitious human alpha - 1 antitrypsin gene expression

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Insert

hAAT (SERPINA1 Human)

Use

Lentiviral

Promoter

CMVp

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pQCascade

Plasmid

#140622

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNA

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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CAG-SaCas9-WPRE

Plasmid

#89996

Purpose

Expresses high levels of WT SaCas9 for genome editing

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

Insert

SaCas9 (NEWENTRY Staphylococcus aureus)

Tags

3xHA, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-ABE5

Plasmid

#195371

Purpose

dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x8

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

ecTadA8e-(GGGGS)x8-dLbCas12a-D156R

Use

CRISPR; Gateway compatible ectada8e-(ggggs)x8-dlb…

Tags

5' and 3' NLS

Expression

Plant

Promoter

ZmUBI1

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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