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BKbeta2 K+ channel scFv [N53/32]

Plasmid

#190561

Purpose

Mammalian Expression of BKbeta2 K+ channel scFV. Derived from hybridoma N53/32.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

BKbeta2 K+ channel (Mus musculus) recombinant scFV (Kcnmb2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE-RPS14(WT)-Myc

Plasmid

#122235

Purpose

expresses RPS14 protein with a Myc tag in mammalian cells (puro resistance)

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RPS14 (RPS14 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

vector's LTR

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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c-Fos scFv [N486/76]

Plasmid

#190560

Purpose

Mammalian Expression of c-Fos scFV. Derived from hybridoma N486/76.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

c-Fos (Homo sapiens) recombinant scFV (FOS Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-NSD1 [N312/10]

Plasmid

#190311

Purpose

Mammalian Expression Plasmid of anti-NSD1 (Human). Derived from hybridoma N312/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-NSD1 (Homo sapiens) recombinant Mouse monoclonal antibody (NSD1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-WRN [N428/12R]

Plasmid

#206575

Purpose

Mammalian Expression Plasmid of anti-WRN (Human). Derived from hybridoma N428/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-WRN (Homo sapiens) recombinant Mouse monoclonal antibody (WRN Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-ACSL3-sfGFP(C) HDR template

Plasmid

#129413

Purpose

HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

ACSL3 HDR template (ACSL3 Human)

Use

CRISPR and TALEN; Endogenous tagging hdr template

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

Plasmid

#162877

Purpose

Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Insert consists of AA1-170

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Kir6.2 K+ channel scFv [N363/71]

Plasmid

#190546

Purpose

Mammalian Expression of Kir6.2 K+ channel scFV. Derived from hybridoma N363/71.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir6.2 K+ channel (Rattus norvegicus) recombinant scFV (Kcnj11 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Calbindin scFv [L109/57]

Plasmid

#182071

Purpose

Mammalian Expression of Calbindin scFv. Derived from hybridoma L109/57.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Calbindin (Homo sapiens) (CALB1 Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-ATF4 [N360A/24]

Plasmid

#190315

Purpose

Mammalian Expression Plasmid of anti-ATF4 (Human). Derived from hybridoma N360A/24.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-ATF4 (Homo sapiens) recombinant Mouse monoclonal antibody (ATF4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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