We narrowed to 4,045 results for: golden gate
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Plasmid#158514PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-6xHA. Km plant selection marker.DepositorAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only
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pIIS18-BufAI-2
Plasmid#128651PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BufAI-1
Plasmid#128650PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA19
Plasmid#158497PurposeIn planta gene expression of Golden gate compatlible A. thaliana ZAR1 L17E. Km plant selection marker.DepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA35
Plasmid#158513PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-6xHA. Km plant selection marker.DepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA33
Plasmid#158511PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1-6xHA. Km plant selection marker.DepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA20
Plasmid#158498PurposeIn planta gene expression of Golden gate complatible A. thaliana ZAR1 D498V/L17E. Km plant selection marker.DepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA41
Plasmid#158519PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1-eGFP. Km plant selection marker.DepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA43
Plasmid#158521PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-eGFP. Km plant selection marker.DepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA42
Plasmid#158520PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-eGFP. Km plant selection marker.DepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA34
Plasmid#158512PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-6xHA. Km plant selection marker.DepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA28
Plasmid#158506PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-4xMyc. Km plant selection marker.DepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA25
Plasmid#158503PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1-4xMyc. Km plant selection marker.DepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0015
Plasmid#117551PurposeLevel 1 Golden Gate Cassette: SaCas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT271.361
Plasmid#127846Purposeclone A3A-W98Y CBEs by SapI Golden GateDepositorInsertA3A-W98Y
MutationW98YAvailable SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0011
Plasmid#117547PurposeLevel 1 Golden Gate Cassette: eCas9 1.1 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + Sp-eCas9 1.1 (no stop codon) (pEPOR0SP0019 + YFP (pICSL50005) + Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0010
Plasmid#117546PurposeLevel 1 Golden Gate Cassette: eCas9 1.0 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + Sp-eCas9 1.0 (no stop codon) (pEPOR0SP0017) + YFP (pICSL50005) + Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0009
Plasmid#117545PurposeLevel 1 Golden Gate Cassette: Cas9-K855A expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-KA (no stop codon)(pEPOR0SP0015) + YFP (pICSL50005)+ Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0008
Plasmid#117544PurposeLevel 1 Golden Gate Cassette: Cas9-DE expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-DE (no stop codon)(pEPOR0SP0001) + YFP (pICSL50005)+ Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0002
Plasmid#117543PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-p (no stop codon)(pEPOR0SP0009) + YFP (pICSL50005)+ Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP2390 [pNP2322 - ISCro4 Donor + ISCro4 Target-RTG-Matched]
Plasmid#247252PurposeMeasure ISCro4 activity via mCherry expressionDepositorInsertpNP2322 - ISCro4_Donor, ISCro4_Target_RTG-Matched
ExpressionMammalianAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)
Plasmid#181792PurposeExpresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNP1723
Plasmid#222727PurposesfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)DepositorTypeEmpty backboneTagssfGFPExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP2270
Plasmid#222733PurposepNP1950 - J23119-IS621 bRNA1 TBL-lacZ-1 (Extended RTG), DBL-WT, HDV; T7-IS621 RecombinaseDepositorInsertIS621 Helper (lacZ)
ExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP2389 [pNP2314 - ISCro4 Donor + ISCro4 Target-RTG-Matched]
Plasmid#247251PurposeMeasure ISCro4 activity via luciferase expressionDepositorInsertpNP2314 - ISCro4_Donor, ISCro4_Target_RTG-Matched
ExpressionMammalianAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
Reckleen_2_SpGCas9
Plasmid#233457PurposeLevel 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniaeDepositorInsertLevel 2 RECKLEEN plasmid
UseCRISPRAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132B2.0
Plasmid#99888PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU3Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131B2.0
Plasmid#99885PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU3Available SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2
Plasmid#182352PurposePan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNP2922 [pNP2321 - Cloning backbone, bRNA Landing Pad, with Ef1a-ISCro4 Recombinase]
Plasmid#247255PurposeCloning backbone for cloning bridge RNAs for ISCro4DepositorTypeEmpty backboneExpressionMammalianAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNP3620 [pNP2916 - ISCro4 bRNA (TBL2, WT + DBL1, WT)]
Plasmid#247267PurposeExpress ISCro4 bridge RNADepositorInsertpNP2916 - ISCro4 bRNA1-179 (WT, TBL2 + DBL1)
ExpressionMammalianAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-tRNA2.0
Plasmid#158393PurposeGolden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNP2314 [Cloning backbone, Gluc/Cluc Inversion Activity Reporter]
Plasmid#247248PurposeCloning backbone for measuring bridge recombinase mediated inversionDepositorTypeEmpty backboneExpressionMammalianAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNP2885 [pNP2922 - ISCro4 TBL1, WT]
Plasmid#247253PurposeExpress ISCro4 recombinase and bridge RNADepositorInsertpNP2922 - ISCro4 bRNA35-100 (TBL1)
ExpressionMammalianAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNP3413 [pNP2922 - ISCro4 TBL2 (WT) + ISCro4 Recombinase (WT)]
Plasmid#247260PurposeExpress ISCro4 recombinase and bridge RNADepositorInsertpNP2922 - ISCro4 bRNA1-100 (TBL2)
ExpressionMammalianAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNP3396 [pNP2922 - ISCro4 DBL1 (WT) + ISCro4 Recombinase (WT)]
Plasmid#247259PurposeExpress ISCro4 recombinase and bridge RNADepositorInsertpNP2922 - ISCro4 bRNA112-179 (DBL1)
ExpressionMammalianAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV GG hUbV-EGFP
Plasmid#216161PurposeContains a Golden-Gate cloning cassette to express up to four gRNA and EGFPDepositorTypeEmpty backboneUseLentiviralAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-tRNA2.0
Plasmid#158394PurposeGolden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-tRNA2.0
Plasmid#158395PurposeGolden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-tRNA2.0
Plasmid#158396PurposeGolden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135-tRNA2.0
Plasmid#158397PurposeGolden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136-tRNA2.0
Plasmid#158398PurposeGolden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFlp-Tc
Plasmid#222336PurposeExpresses Flp and tetracycline to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-TetAR-pBBR1-ELT3DepositorAvailable SinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF4)-TS.FF4x2-bGH
Plasmid#235294PurposeComMAND closed-loop circuit regulating mRuby2 with 2 copies of microRNA target sitesDepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNP1954
Plasmid#222730PurposepNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)DepositorInsertIS621 bridge RNA
TagssfGFPExpressionBacterialAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mAmetrine
Plasmid#228598PurposeMammalian expression of mAmetrine, for MuSIC barcode constructionDepositorInsertmAmetrine
ExpressionMammalianPromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132A2.0
Plasmid#99887PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU6Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133A2.0
Plasmid#99891PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU6Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
miRFP670-TRF1
Plasmid#227388PurposeLabel telomere, no adhesionDepositorInsertTelomeric miRFP (TERF1 Human)
UseLentiviralAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP568
Plasmid#222725PurposepNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cisDepositorInsertIS621 bridge RNA
TagssfGFPExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only