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Plasmid#14481DepositorInsertFLIPmal
UseTagsECFP and EYFPExpressionBacterialMutationW62APromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPmal-control
Plasmid#14482DepositorInsertFLIPmal
UseTagsECFP and EYFPExpressionBacterialMutationW340APromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSET A-mEosEM-E
Plasmid#226336PurposePhotoswitchable fluorescent protein for correlative light-electron microscopy; mEosEM-E for prokaryotic expressionDepositorInsertmEosEM-E
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK652
Plasmid#72470PurposeProduces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)DepositorInsertacetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationMutated histidine-233 to glycinePromoterT7Available sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mCherry_Grx1_roGFP2
Plasmid#155045PurposeFluorescent redox reporterDepositorInsertGlutaredoxin-1 (GLRX Human)
UseLentiviralTagsroGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-tTA
Plasmid#104102PurposeExpresses tTA2 under the CAG promoter in mammalian cells.DepositorInserttTA2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-FLAG
Plasmid#119759PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.DepositorTypeEmpty backboneUseTagsFLAG and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-FR-GECO1c
Plasmid#163682PurposeFar-red fluorescent calcium indicator FR-GECO1cDepositorInsertFR-GECO1c
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-LUCI-GECO1
Plasmid#113675PurposeGenetically-encoded ratiometric bioluminescent Ca2+ indicator based on GCaMP6sDepositorInsertLUCI-GECO1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Malibu-mScarlet
Plasmid#229554PurposeFluorescent reporter for malonyl-CoADepositorInsertMalibu-mScarlet
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-His
Plasmid#119755PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.DepositorTypeEmpty backboneUseTags10xHis and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-FR-GECO1a
Plasmid#163681PurposeFar-red fluorescent calcium indicator FR-GECO1aDepositorInsertFR-GECO1a
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
PJEBAN6
Plasmid#203684PurposeExpresses red fluorescent marker DsRed-Express in Listeria monocytogenesDepositorInsertDsRed-Express
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC101-AmpR EV
Plasmid#208184Purposeempty plasmid vector, non-fluorescentDepositorTypeEmpty backboneUseReporterTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
ampkar
Plasmid#35097PurposeA fluorescent reporter of AMPK activity and cellular energy stressDepositorInsertampkar
UseTagsECFP and VenusExpressionBacterialMutationnaPromoterCMVAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTW357bN
Plasmid#227932PurposeAccessory plasmid for negative selectionDepositorInsertsgIII-neg
T7c
UseTagsExpressionBacterialMutationR632SPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-Strep2
Plasmid#119758PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.DepositorTypeEmpty backboneUseTagsGST and Strep-tag II fusion tagExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-A96 ELP
Plasmid#67856PurposeGFP Fluorescent Elastin-like Polypeptide(VPGAG) with 96 ELP repeats containing Alanine guest residueDepositorInsertGFP fused to ELP (VPGAG) repeated 96 times
UseTagsGFP fused to N-terminal of ELPExpressionMammalianMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
GW1CMV-Perceval
Plasmid#21737Purposecan be used to monitor the ATP:ADP ratio during live-cell imagingDepositorInsertPerceval (fluorescent ATP:ADP sensor)
UseTags5'UTRExpressionMammalianMutationimproved Kozak sequencePromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGAL-GFP kan
Plasmid#122060Purposegalactose inducible expression of GFP in yeast. marked with URA3DepositorInsertgreen fluorescent protein
UseTagsExpressionYeastMutationPromoterGAL1Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only