162,717 results

1. pGEXTK-Pak1 70-117

   Plasmid

   #12217

   Depositor

   Jonathan Chernoff

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   PBD (p21-binding domain), aa 70-117

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. amajLime chromoprotein

   Plasmid

   #117843

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amajLime
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pG106 - KA

   Plasmid

   #178041

   Purpose

   Bacteroides - Escherichia coli shuttle vector with ErmF and ErmAM, Kan and Amp selection markers

   Depositor

   Janina Lewis

   Article

   Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

   Type

   Empty backbone

   Use

   Shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. dCas9-CBP core

   Plasmid

   #179543

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENTR-NLS-actin

   Plasmid

   #118380

   Purpose

   Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Tags

   Expression

   Mutation

   NLS

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEYFP-NR1a

   Plasmid

   #17928

   Depositor

   Stefano Vicini

   Article

   Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

   Insert

   N-methyl-D-aspartate receptor NMDAR1 (Grin1 Rat)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBJG1 T7-8xHis-OGT

   Plasmid

   #154271

   Purpose

   Bacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Gross et al J Am Chem Soc. 2005 Oct 26;127(42):14588

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   bacterial codon optimized

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQE30-butelase1

   Plasmid

   #115430

   Purpose

   Expresses the macrocyclizing asparaginyl endopeptidase butelase 1 in E. coli

   Depositor

   Joshua Mylne

   Article

   James et al Plant J. 2019 Feb 21. doi: 10.1111/tpj.1

   Insert

   butelase 1
   

   Use

   Tags

   6-His

   Expression

   Mutation

   Promoter

   T5

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAcGFP-Hyg-CTCF-N1

   Plasmid

   #131799

   Purpose

   Expresses human CTCF-GFP in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF2858

    Plasmid

    #143385

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ID3 (ID3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge