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pAAV-CAG-FRT-Rev-3xGFP

Plasmid

#191204

Purpose

FlpO dependent GFP expression

Depositor

Janelia Viral Tools

Article

Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

Insert

3X GFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SpCas9D10A nickase

Plasmid

#216737

Purpose

Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)

Depositor

Vincent Dion

Article

Alvaro et al bioRxiv

Insert

Cas9-D10A nickase

Use

AAV and CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

CMVd1

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINB-HOIL-1 full-length (1–510)

Plasmid

#193858

Purpose

Expression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coli

Depositor

Bernhard Lechtenberg

Article

Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:

Insert

HOIL-1 (RBCK1 Human)

Tags

3C protease cleavage site and 6x-His tag

Expression

Bacterial

Mutation

Codon optimised for expression in E. coli

Promoter

T7

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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iChr2-Notch Mosaic (SO250)

Plasmid

#99752

Purpose

Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteins and Notch signalling genes

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

HIs-H2B-Cherry, H2B-EGFP-V5, HA-H2B-Cerulean, DN-Maml1, NICD-PEST

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLX304-ARNT-d414

Plasmid

#203579

Purpose

Expresses a functionally inactive version of ARNT with 5’ 414 bp deletion [Δ414] in mammalian cells.

Depositor

Abhishek Chakraborty, William Kaelin

Article

Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222

Insert

ARNT (ARNT Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

5' 414 basepair deletion [Δ414]

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b-FNLDTA

Plasmid

#195340

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Article

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis Tag and MBP, TEV cleavage site, 6x His Tag

Expression

Bacterial

Mutation

F208W, N524V, L795I, D868V, T874S, A1015E

Promoter

T7 Promoter

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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anti-Desmin_D7-pBIOCAM5

Plasmid

#39346

Depositor

John McCafferty

Article

Schofield et al Genome Biol. 2007;8(11):R254.

Insert

scFv-Fc-fusion specific for Desmin (clone D7) (DES Human)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-7xTRE-3xeGFP

Plasmid

#191206

Purpose

GFP expression under the control of TRE promotor

Depositor

Janelia Viral Tools

Article

Glaser et al Nat Methods. 2022 May;19(5):613-619. doi

Insert

3X GFP

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2

Plasmid

#112668

Purpose

Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression of your protein of interest.

Depositor

Catherine Verfaillie

Article

Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

Insert

tdT

Use

AAV; Donor plasmid for recombinase-mediated casse…

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pBMN DHFR-HiBiT / Lyt2

Plasmid

#179731

Purpose

Expresses human dihydrofolate reductase (DHFR) with C-terminal HiBiT tag in mammalian cells. This retroviral vector has an IRES-Lyt-2 downstream of the cloning sites

Depositor

James Inglese

Article

Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23

Insert

human DHFR-HiBiT and IRES-Lyt-2 (DHFR Human, Mouse)

Use

Retroviral

Tags

HiBiT

Promoter

LTR/MMLV

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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