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pTS1107_Tier3(SB)

Plasmid

#169634

Purpose

Tier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase (5'ITR-A1-pA::A2-pA::A3-pA-3'ITR)

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAM110

Plasmid

#86480

Purpose

Expresses p12 and p9-6His-tnaA

Depositor

Pamela Silver

Publication

Myhrvold et al ACS Synth Biol. 2016 Aug 3.

Insert

p12, p9-6His-tnaA

Use

Synthetic Biology

Tags

6His and tnaA

Expression

Bacterial

Promoter

pBAD

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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PHP11004

Plasmid

#132353

Purpose

R over-expression cassette for Zea mays

Depositor

Joshua Young

Publication

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

R

Expression

Plant

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSL1103

Plasmid

#129526

Purpose

pDEF-SpCas9, No Target Control Vector for CRISPRi, PbEF1a promoter, 1st Generation

Depositor

Scott Lindner

Publication

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

dSpCas9

Use

Plasmodium, rodent-infectious species

Tags

NLS, 1xHA

Available since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL11085_GG_Wheat_live_Cas9_for

Plasmid

#126881

Purpose

Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.

Depositor

Sophien Kamoun

Insert

Wheat_live_Cas9

Use

CRISPR

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1106

Plasmid

#119249

Purpose

construction intermediate

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pOCC110

Plasmid

#118905

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)

Depositor

Marc Bickle, PEPC MPI CBG

Publication

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-3C-NotI-ccdB-AscI-LPETGg-stop-HindIII cassette

Tags

HIS6, cleavable with 3C protease and LPETGg

Expression

Insect

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCR1110

Plasmid

#111096

Purpose

Expresses BLM_+_91260645.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Publication

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

BLM gRNA (BLM Human)

Use

Lentiviral

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL11061

Plasmid

#68255

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleracea

Depositor

Nicola Patron

Publication

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_AtU6-26 + sgRNA_BolC.GA4a-1

Use

Synthetic Biology

Expression

Plant

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL11058

Plasmid

#68254

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in barley

Depositor

Nicola Patron

Publication

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_TaU6 + sgRNA_HvPM19_3

Use

Synthetic Biology

Expression

Plant

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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TAL3110

Plasmid

#41284

Depositor

Keith Joung

Publication

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-lyl1-Left (lyl1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
pEMS1100

Plasmid

#29031

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple232 (TNNT1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1106

Plasmid

#29035

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple171 (RAMP3 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1107

Plasmid

#29036

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple172 (RAMP3 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEMS1102

Plasmid

#29033

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748)

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple183 (S100B Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf110

Plasmid

#12798

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Publication

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf110

Expression

Mammalian

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

Plasmid

#169336

Purpose

Expression of the LT-Fucci(SA) in mammalian cells

Depositor

Kai Johnsson

Publication

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(SA) (GMNN, CDT1 Human)

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Violet

Plasmid

#160447

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Violet chromoprotein in E. coli

Depositor

Anthony Forster

Publication

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Violet

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-3 [N110/29.4R]

Plasmid

#114518

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-3 (Rat). Derived from hybridoma N110/29.4.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neuroligin-3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn3 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-saCBE_KKH C-terminal

Plasmid

#137184

Purpose

AAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE, and U6-sgRNA (KKH variant).

Depositor

David Liu

Publication

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-saCBE_KKH C-terminal

Use

AAV

Mutation

E782K;N968K;R1015H conferring recognition of NNNR…

Promoter

Cbh

Available since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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