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pRH3243

Plasmid

#210187

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33P-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::aro7-R33P-GFP::tADH1 (ARO7 Budding Yeast)

Expression

Yeast

Mutation

R33P

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 bleach.2

Plasmid

#191878

Purpose

Encodes a GFP design, bleach.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.2

Tags

His Tag

Expression

Bacterial

Mutation

V61L T65S V68A S72A V112I Y145M

Promoter

T7

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUBQ10:LLFLS2-1

Plasmid

#202187

Purpose

Express 'Frost Lisbon' Lemon FLS2-1 gene under the UBQ10 promoter

Depositor

Gitta Coaker

Article

Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

Insert

Flagellin sensing 2

Tags

HA

Expression

Plant

Promoter

AtUBQ10

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 bleach.6

Plasmid

#191879

Purpose

Encodes a GFP design, bleach.6

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.6

Tags

His Tag

Expression

Bacterial

Mutation

V61L S72C Y145M H181L V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 slow.3

Plasmid

#191877

Purpose

Encodes a GFP design, slow.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

slow.3

Tags

His Tag

Expression

Bacterial

Mutation

Q69L S72V Y145S T167V L220V V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NLS-QPAS1-VP16

Plasmid

#186187

Purpose

AAV vector packaging QPAS1-VP16, a component of optogenetic system for NIR light-controllable transcriptional activation

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2022 May 19;13(1):2813. doi:

Insert

NLS-QPAS1-VP16

Use

AAV

Promoter

CAG

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRS315Nab3D191stop

Plasmid

#110537

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al PLoS One. 2017 Oct 12;12(10):e0186187. d

Insert

NAB3 (NAB3 Budding Yeast)

Expression

Yeast

Mutation

C-terminal deltion of 191 amino acids

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-Tet-off-uniRapR-mCherry-Vav2(DPZ)

Plasmid

#91874

Purpose

Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2 (VAV2 Human)

Tags

mCherry

Expression

Bacterial and Mammalian

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.16.2

Plasmid

#98187

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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TAL3468

Plasmid

#47187

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-kcnma1a-left (kcnma1a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
pDMG10c

Plasmid

#33187

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

aex-4/tag-81 3'UTR (tag-81, aex-4 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

LTA site: atacGaT

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDG458

Plasmid

#100900

Purpose

SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

Depositor

Paul Thomas

Article

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh and U6

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-His6-2_HBD

Plasmid

#171870

Purpose

Expresses tandem repeats of HBD domain of RNase H1 for better DNA-RNA Hybrid recognition specificity in bacterial cells

Depositor

Kaiwei Liang

Article

Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

Insert

Tandem repeats Hybrid binding domain of Rnase H1

Tags

GST- and His10-tag

Expression

Bacterial

Mutation

WT

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pTight-hASCL1-N174

Plasmid

#31876

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

achaete-scute complex homolog 1 (ASCL1 Human)

Use

Lentiviral

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti HRE-Luc pGK Hygro

Plasmid

#118706

Purpose

reporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferase

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

HRE (HIF-responsive promoter (3xHRE)-luc

Use

Lentiviral

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-IFT20

Plasmid

#218719

Purpose

Expresses C-terminally EGFP-tagged IFT20 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT20 (IFT20 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr

Plasmid

#218767

Purpose

encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG

Depositor

Abhishek Chatterjee

Article

Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

Insert

EcTyrRS-pAAFRS-9

Expression

Bacterial

Mutation

Y37G, L71V, D182C, F183Y, L186C, D265R

Promoter

tacI

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pDG330

Plasmid

#100898

Purpose

SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

Depositor

Paul Thomas

Article

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh and U6

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Cas12aUltra_5XNLS

Plasmid

#218775

Purpose

His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS

Depositor

Ross Wilson

Article

Sahu et al bioRxiv 2024.07.14.603391

Insert

5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a

Use

CRISPR

Tags

6XHis (C terminal), HA, Nucleoplasmin, SV40, and …

Expression

Bacterial

Mutation

Nucleotides mutations were made to encode for arg…

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-control-APEX

Plasmid

#73187

Purpose

Expresses "control"-APEX (cytosolic) in mammalian cells

Depositor

Maxence Nachury

Article

Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

Insert

control-APEX (Nphp3 Mouse, Glycine max)

Tags

internal GFP tag

Expression

Mammalian

Mutation

changed glycine 2 to alanine

Promoter

EF1alpha

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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