We narrowed to 23,600 results for: CHI;
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Plasmid#189848PurposeIntron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 3.DepositorTypeEmpty backboneUseCre/LoxTagsmRFP and tagBFPExpressionMutationPromoterbeta ActinAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only
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pAID1.2-EF1a-NmClover3-hsLem2
Plasmid#179524PurposeExpress OsTIR1 and mClover3-mAID-hsLem2 in mammalian cells under the control of EF1a promoterDepositorInsertLEMD2 (LEMD2 Human)
UseTagsmClover3-mAIDExpressionMammalianMutationPromoterEF1αAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
NKP9 pmRFP-N2 (719)
Plasmid#61999Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertC17orf62 (CYBC1 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
NKP40 RFP (794)
Plasmid#62006Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertC17orf32 (C17orf32 Human)
UseTagsmRFPExpressionMammalianMutationPromoterpgkAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHP-2(NC)-SH2
Plasmid#46499DepositorInsertSHP-2(NC) (PTPN11 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-222PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF[TAG]
Plasmid#164579PurposeMachinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codonsDepositorInsertsMj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]
UseTagsExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoteraraBAD, glnS, and proKAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHP-2(N)-SH2
Plasmid#46498DepositorInsertSHP-2(N) (PTPN11 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-104PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX RASGAP(N)-SH2
Plasmid#46432DepositorInsertGap/RASGAP(N) (RASA1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 168-283PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHP-2(C)-SH2
Plasmid#46497DepositorInsertSHP-2(C) (PTPN11 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 101-222PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX RASGAP(C)-SH2
Plasmid#46431DepositorInsertGap/RASGAP(C) (RASA1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 339-452PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX RASGAP(NC)-SH2
Plasmid#46433DepositorInsertGap/RASGAP(NC) (RASA1 Human)
UseTagsGSTExpressionBacterialMutationcontains amino acids 171-448PromoterTacAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
NET23 pEGFP-N2 (1174)
Plasmid#62037Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET23 (STING1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT2K-p53DN-T2A-copGFP
Plasmid#109229PurposeFor Tol2 transposon-mediated stable expression of dominant negative p53 and copGFPDepositorInsertdominant negative p53 (Trp53 Mouse)
UseTagscopGFPExpressionMammalianMutationdominant negative p53PromoterCAGGSAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDL52 (clone45)
Plasmid#188578PurposeHigh-yield production of coenzyme F420 in E. coliDepositorInsertsribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE
UseTagsExpressionBacterialMutationCodon optimization, synthetic ribosome binding si…PromoterT7 variantsAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDL52 (clone76)
Plasmid#188579PurposeHigh-yield production of coenzyme F420 in E. coliDepositorInsertsribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE
UseTagsExpressionBacterialMutationCodon optimization, synthetic ribosome binding si…PromoterT7 variantsAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2K-LRL-mouse c-MYC-IRES-Luc2
Plasmid#109228PurposeFor Tol2 transposon-mediated stable expression of LRL, c-Myc and IRES-luciferaseDepositorInsertc-Myc (Myc Mouse)
UseTagsIRES-Luc2ExpressionMammalianMutationPromoterCAGGSAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZc-HsACTG1*-thymosinB-8His
Plasmid#111147PurposeExpresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.DepositorInsertactin gamma 1 (ACTG1 Human)
UsePichia pastoris integration vectorTagsThymosin beta and a His-tagExpressionYeastMutationcodon-optimised for expression in Pichia pastorisPromoterAOX1Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBbleo-TGFa-EREG-ScNeo
Plasmid#209915PurposeTo express the chimeric protein of TGFα and EREG, which a recombinant TGFα protein fused extracellularly to the mScarlet and a recombinant Epiregulin protein fused intracellularly to the mNeonGreenDepositorUseTagsGGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…ExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4
Plasmid#193294Purposeepisomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversionDepositorUseTagsExpressionMammalianMutationSOX2-17 is engineered highly cooperative chimeric…PromoterCAGAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-SOX2-17-p2a-KLF4
Plasmid#193290Purposeepisomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversionDepositorUseTagsExpressionMammalianMutationSOX2-17 is engineered highly cooperative chimeric…PromoterCAGAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only