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CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

Plasmid

#107307

Purpose

Encoding an SPH activation system

Depositor

Hui Yang

Publication

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Insert

CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA

Expression

Mammalian

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Flag-Axin1

Plasmid

#109370

Purpose

Transient over-expression of human Axin1

Depositor

Mariann Bienz

Publication

Fiedler et al Proc Natl Acad Sci U S A. 2011 Feb 1;108

Insert

Axin1 (AXIN1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFGAMS-EGFP

Plasmid

#99107

Purpose

Expresses human PFAS-EGFP fusion in mammalian cells

Depositor

Stephen Benkovic

Publication

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

PFAS (PFAS Human)

Tags

EGFP (enhanced green fluorescent protein)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Lck-mTurquoise2

Plasmid

#98822

Purpose

In vivo visualization of the plasma membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lck

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pET42b-BE3

Plasmid

#87437

Purpose

Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Publication

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

BE3 (Apobec1 Human, Rat, Bacillus phage PBS2)

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET42b-HF-BE3

Plasmid

#87438

Purpose

Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Publication

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

HF-BE3 (Apobec1 Human, Rat, Bacillus phage PBS2)

Tags

His6

Expression

Bacterial

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmScarlet-i_Giantin_C1

Plasmid

#85050

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Giantin (GOLGB1 Human)

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-N1-human lyn–GFP

Plasmid

#35958

Purpose

Expresses human lyn protein codon optimized for zebrafish expression.

Depositor

Anna Huttenlocher

Publication

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

LYN (LYN Human)

Tags

EGFP

Expression

Mammalian

Mutation

wt

Promoter

cmv

Available since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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GFP- RPL26

Plasmid

#31980

Depositor

Michael Kastan

Publication

Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

Insert

RPL26 (RPL26 Human)

Tags

GFP

Expression

Mammalian

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CFP-C1-PLCdelta-PH

Plasmid

#21262

Depositor

Tobias Meyer

Publication

Botelho et al J Cell Biol. 2000 Dec 25. 151(7):1353-68

Insert

PLCdelta (PLCD1 Human)

Tags

CFP

Expression

Mammalian

Mutation

PH domain of PLCdelta (human). Also known as PLCd…

Available since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEGFP-GIT1

Plasmid

#15226

Depositor

Rick Horwitz

Publication

Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

Insert

G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)

Tags

GFP

Expression

Mammalian

Mutation

L425V, E476K and L583P

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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LAMP1-RpHLuorin2

Plasmid

#171720

Purpose

Lysosomal expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Publication

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-Frankenbody-FLAG-mRuby2

Plasmid

#175294

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Publication

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mRuby2

Tags

mRuby2

Expression

Mammalian

Available since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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GFP-Rab27B

Plasmid

#89447

Purpose

Expression of GFP-tagged human Rab27B in mammalian cells

Depositor

Wendy Westbroek

Publication

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pH2B-miRFP703

Plasmid

#80001

Purpose

Histone 2B labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

histone cluster 1 H2B family member j (H2BC11 Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pEYFP-Sec24C

Plasmid

#66608

Purpose

YFP fusion of Sec24C

Depositor

David Stephens

Publication

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec24C (SEC24C Human)

Expression

Mammalian

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Ai65(RCFL-tdT) targeting vector

Plasmid

#61577

Purpose

Target a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

Depositor

Hongkui Zeng

Publication

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

pCAG-FSF-LSL-tdTomato

Use

Cre/Lox and Mouse Targeting

Promoter

CAG

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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YFP NLS Beta-Actin

Plasmid

#60613

Purpose

Encodes YFP and NLS tagged beta-actin

Depositor

Primal de Lanerolle

Publication

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mCherry-Calnexin-N-14

Plasmid

#55005

Purpose

Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Calnexin (CANX Human)

Tags

mCherry

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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dClover2-C1

Plasmid

#54577

Purpose

Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

Depositor

Michael Davidson

Publication

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Type

Empty backbone

Tags

dClover2

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

10 Articles

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