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Showing: 1321 - 1340 of 1671 results

  1. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

    Plasmid
    #50417
    Purpose
    Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.
    Depositor
    Andrew Scharenberg
    Inserts
    eGFP with I-Sce I TS
    +3 mCherry
    Use
    Lentiviral
    Tags
    T2A dislinker and iRFP
    Expression
    Mammalian
    Mutation
    embedded I-Sce I TS from 163-185
    Promoter
    SFFV
    Available since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    50417-plasmid-map-sequence-id-75349

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  2. pKG212

    Plasmid
    #69758
    Purpose
    Retroviral expression of CENP-T aa 1-242
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-242 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and mCherry
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Promoter
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pET24a-LaM4-3xTS

    Plasmid
    #162777
    Purpose
    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    anti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and TS site from proCCK
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162777-plasmid-map-sequence-id-321882

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  5. pET24a-LaM4-1xTS

    Plasmid
    #162778
    Purpose
    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    anti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and TS site from proCCK
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162778-plasmid-map-sequence-id-321887

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  7. pET24a-LaM4-2xTS

    Plasmid
    #162779
    Purpose
    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    anti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and TS site from proCCK
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162779-plasmid-map-sequence-id-321884

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  8. p37-2iDMD-LR

    Plasmid
    #88892
    Purpose
    37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    88892-plasmid-map-sequence-id-451853

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  9. p28-2iDMD-puro

    Plasmid
    #88895
    Purpose
    28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    puromycin resistance enzyme
    mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    88895-plasmid-map-sequence-id-451851

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  10. Myc var1 K51/52 to NQ

    Plasmid
    #127287
    Purpose
    Expresses Myc with putative Ub site mutated
    Depositor
    Christine Milcarek
    Insert
    Myc (NM_001177352) (Myc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K51 and 52 to N and Q by Q5 mutagenesis
    Promoter
    CMV
    Available since
    Nov. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    127287-plasmid-map-sequence-id-250022

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  11. Myc var 1 K396/397 to NQ

    Plasmid
    #127286
    Purpose
    Expresses Myc with putative Ub site mutated
    Depositor
    Christine Milcarek
    Insert
    Myc (NM_001177352) (Myc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K396 and 397 to N and Q by Q5 mutagenesi
    Promoter
    CMV
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127286-plasmid-map-sequence-id-249993

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  12. pcDNA3-endo-Nb80

    Plasmid
    #205518
    Purpose
    Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    endo-Nb80
    Use
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205518-plasmid-map-sequence-id-409484

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  13. pcDNA3-endo-Nb37

    Plasmid
    #205514
    Purpose
    Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    endo-Nb37
    Use
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205514-plasmid-map-sequence-id-409423

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  14. pcDNA3-nuc-Nb80

    Plasmid
    #205519
    Purpose
    Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    nuc-Nb80
    Use
    Tags
    Nuclear localization signal (NLS) and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205519-plasmid-map-sequence-id-409422

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  15. pcDNA3-nuc-Nb37

    Plasmid
    #205515
    Purpose
    Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    nuc-Nb37
    Use
    Tags
    Nuclear localization signal (NLS) and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205515-plasmid-map-sequence-id-409490

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  19. RFP-(SIM)3

    Plasmid
    #127148
    Purpose
    Mammalian expression plasmid for mCherry-(SIM)3 client to assess SUMO-SIM scaffolds.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    3 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127148-plasmid-map-sequence-id-255288

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  20. pAS132

    Plasmid
    #211503
    Purpose
    AAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Insert
    mCherry
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211503-plasmid-map-sequence-id-417791

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Showing: 1321 - 1340 of 1671 results