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Atg11 [699-800] pHis2

Plasmid

#190264

Purpose

Codon optimized Atg11 amino acids 699-800 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Margolis et al J Mol Biol. 2020 Oct 2;432(21):5752-5764

Insert

ATG11

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO HNRNPA1 dHexa LCD

Plasmid

#197014

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Molliex et al Cell. 2015 Sep 24;163(1):123-33. doi: 10

Insert

HNRNPA1

Tags

6His-SUMO

Expression

Bacterial

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPEPT1

Plasmid

#195001

Purpose

Production of peptides in E. coli as GB1 fusion

Depositor

Marko Hyvönen

Article

Pantelejevs et al Chem Sci. 2023 Nov 21;14(47):13915-13923

Type

Empty backbone

Tags

His8-tag and StrepTagII-GB1

Expression

Bacterial

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(HcdR)-HcdR

Plasmid

#191648

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), hcdR complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

HcdR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(CadR)-CadR

Plasmid

#191649

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), cadR complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CadR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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T7-10-QUAS-GFP_ColE1

Plasmid

#171657

Purpose

QUAS ten base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-10-QUAS

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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T7-15-QUAS-GFP_ColE1

Plasmid

#171658

Purpose

QUAS fifteen base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-15-QUAS

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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T7-TetO-QF_T7-LacO-TetR_p15A

Plasmid

#171659

Purpose

Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. Contains a p15A origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-LacO-TetR-T7 Stop

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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T7-0-QUAS-GFP_ColE1

Plasmid

#171650

Purpose

QUAS directly downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-0-QUAS

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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QUAS-0-T7-CcdB_ColE1

Plasmid

#171652

Purpose

QUAS directly upstream of a T7 promoter. Expresses CcdB in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

CcdB-ssrA

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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