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pGAL4-DBD-S6Y_AroPATCHY3

Plasmid

#215632

Purpose

For overexpression of Gal4-DBD S6Y AroPATCHY 3

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

S6Y AroPATCHY 3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGal4-DBD-OCT4_WT_C

Plasmid

#215623

Purpose

For overexpression of Gal4-DBD OCT4 WT C

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

OCT4 C

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-S6Y_AroPATCHY1

Plasmid

#215633

Purpose

For overexpression of Gal4-DBD S6Y AroPATCHY1

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

S6Y AroPATCHY1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGal4-DBD-OCT4_AroPERFECT_N

Plasmid

#215624

Purpose

For overexpression of Gal4-DBD OCT4 AroPERFECT N

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

OCT4 AroPERFECT N

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pL0_BC-pyr_comp_sig_pep

Plasmid

#202132

Purpose

Level 0 plasmid containing a kozak consensus sequence and a pyrenoid compartment signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Pyrenoid compartment signal peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Sept. 6, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-ER_sig_pep

Plasmid

#202136

Purpose

Level 0 plasmid containing a kozak consensus sequence and a ER signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Endoplasmic reticulum signal peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-mito_sig_pep

Plasmid

#202134

Purpose

Level 0 plasmid containing a kozak consensus sequence and a mitochondria signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Mitochondria signal peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-sec_sig_pep

Plasmid

#202133

Purpose

Level 0 plasmid containing a kozak consensus sequence and a secretion signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Secretion signal peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-chl_sig_pep

Plasmid

#202131

Purpose

Level 0 plasmid containing a kozak consensus sequence and a chloroplast signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Chloroplast signal peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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FUW-MD-mTurquoise

Plasmid

#193094

Purpose

constitutive expression of the mitosis degradation (MD) domain of cyclin B1 fused to mTurquoise in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

MD-mTurquoise

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-CteIF4E_35-250-del183-202-I87AI91A_AF

Plasmid

#148722

Purpose

Bacterial Expression of CteIF4E_35-250-del183-202-I87AI91A

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4E_35-250-del183-202-I87AI91A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJAK175

Plasmid

#178601

Purpose

pAP259 derived plasmid encoding a xylose inducible BitlucOpt

Depositor

Robert Fagan

Article

Phothichaisri et al Microbiol Spectr. 2022 Apr 4:e0236121. d

Insert

Bitlucopt

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pxyl

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV_Hyg_FLAG-BirA-eGFP-NLS2

Plasmid

#170917

Purpose

Expresses FLAG-BirA-eGFP-NLS2 to be used as control in BioID or FLAG pull down

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

FLAG-BirA-eGFP-NLS2

Use

Tags

BirA-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_HR-BirA-FLAG_Ago2

Plasmid

#170918

Purpose

Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago2

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

BirA-FLAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

lac

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_HR-BirA-FLAG_Ago1

Plasmid

#170919

Purpose

Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago1

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

BirA-FLAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

lac

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-G1pCNPRS

Plasmid

#174719

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-5-pyridyl)alanine (pCNP), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

Insert

tRNA synthetase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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MPC026: pAAV-CAG-FLEX-NovArch-TS-Citrine_TSx3-ER2

Plasmid

#153195

Purpose

encodes pAAV NovArch

Depositor

Adam Cohen

Article

Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

Insert

NovArch

Use

AAV

Tags

citrine

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pL2-1 ConjEpi PtEF2bsd Pt49202tHMGRVenusThrFLAG

Plasmid

#139964

Purpose

Over-expression of N-terminal truncated version of native 3-hydroxy-3-methyl glutaryl CoA reductase

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

ConjEpi PtEF2_bsd_PttEF2 Pt49202_tHMGRVenusThrFLAG_Ptt49202

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP35-TD-HilA

Plasmid

#153332

Purpose

Triggers expression of SptP35 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

HA4-7c12 Tandem Monobody

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP92-TD-HilA

Plasmid

#153331

Purpose

Triggers secretion of SptP92 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP92-HA4-7c12 Tandem Monobody

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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