We narrowed to 11,674 results for: NSI

1. pYSD024

   Plasmid

   #212910

   Purpose

   Encodes the wildtype S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappWT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   MFalpha1 (MF(ALPHA)1 Budding Yeast)
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYSD042

   Plasmid

   #212912

   Purpose

   Encodes the S. cerevisiae HSP150 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   HSP150 (HSP150 Budding Yeast)
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYSD005

   Plasmid

   #212902

   Purpose

   Encodes S. cerevisiae KSH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   KSH1 (KSH1 Budding Yeast)
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYSD044

   Plasmid

   #212914

   Purpose

   Encodes the S. cerevisiae 142 amino acid SCW4 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   SCW4 (SCW4 Budding Yeast)
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYSD022

   Plasmid

   #212908

   Purpose

   Encodes an engineered S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappOPT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   MFalpha1 (MF(ALPHA)1 Budding Yeast)
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYSD009

   Plasmid

   #212906

   Purpose

   Encodes S. cerevisiae SRL1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   SRL1 (SRL1 Budding Yeast)
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. RS02060_pMQ30_KO_Kan

   Plasmid

   #185391

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS02060 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS02060 upstream flanking homology region
   RR42_RS02060 downstream flanking homology region
   

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. RS28935_pMQ30_KO_Kan

   Plasmid

   #185392

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS28935 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS28935 upstream flanking homology region
   RR42_RS28935 downstream flanking homology region
   

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. RS18965_pMQ30_KO_Kan

   Plasmid

   #185394

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS18965 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS18965 upstream flanking homology region
   RR42_RS18965 downstream flanking homology region
   

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. RS18970_pMQ30_KO_Kan

    Plasmid

    #185395

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS18970 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS18970 upstream flanking homology region
    RR42_RS18970 downstream flanking homology region
    

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA4.TO_ORF18_TurboID_3xHA

    Plasmid

    #200017

    Purpose

    Expresses KSHV protein ORF18 fused at the C-terminal to TurboID-3xHA

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    ORF18
    

    Tags

    TurboID-3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA4.TO_ORF30_TurboID_3xHA

    Plasmid

    #200018

    Purpose

    Expresses KSHV protein ORF30 fused at the C-terminal to TurboID-3xHA

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    ORF30
    

    Tags

    TurboID-3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA4.TO_ORF31_TurboID_3xHA

    Plasmid

    #200019

    Purpose

    Expresses KSHV protein ORF31 fused at the C-terminal to TurboID-3xHA

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    ORF31
    

    Tags

    TurboID-3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA4.TO_ORF66_TurboID_3xHA

    Plasmid

    #200020

    Purpose

    Expresses KSHV protein ORF66 fused at the C-terminal to TurboID-3xHA

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    ORF66
    

    Tags

    TurboID-3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA4.TO_3xHA_TurboID_ORF34

    Plasmid

    #200021

    Purpose

    Expresses KSHV protein ORF34 fused at the N-terminal to 3xHA-TurboID

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    ORF34
    

    Tags

    3xHA-TurboID

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJM1-zeo G226A ORF29

    Plasmid

    #200024

    Purpose

    Lentiviral vector for expression of KSHV ORF29 with G226A mutation

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    ORF29
    

    Use

    Lentiviral

    Mutation

    Glycine 226 changed to alanine

    Promoter

    CMV

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUBQ10:WNFLS2-2

    Plasmid

    #202190

    Purpose

    Express 'Washington navel' Orange FLS2-2 gene under the UBQ10 promoter

    Depositor

    Gitta Coaker

    Article

    Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

    Insert

    Flagellin sensing 2
    

    Tags

    HA

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUBQ10:WNFLS2-1

    Plasmid

    #202189

    Purpose

    Express 'Washington navel' Orange FLS2-1 gene under the UBQ10 promoter

    Depositor

    Gitta Coaker

    Article

    Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

    Insert

    Flagellin sensing 2
    

    Tags

    HA

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUE1-TSP-ORF68 R14A/K310A

    Plasmid

    #162655

    Purpose

    Expresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acids R14 and K310 mutated to alanine

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLJM1-zeo-2xStrep-ORF68 C52A

    Plasmid

    #162660

    Purpose

    Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 C52A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Lentiviral

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid C52 mutated to alanine

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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