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Showing: 1341 - 1360 of 29406 results

  1. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid
    #174091
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV and T7
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174091-plasmid-map-sequence-id-341917

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  2. CYP51A1 (3I3K)

    Plasmid
    #25161
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CYP51A1_cw_09 (CYP51A1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 54-502
    Promoter
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. 6CDG (GID4)

    Plasmid
    #119961
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    GID4 (C17orf39 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 124-289
    Promoter
    T7
    Available since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119961-plasmid-map-sequence-id-232684

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  5. pARNTL.1.0-gDNA

    Plasmid
    #112480
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ARNTL
    Depositor
    Kevin White
    Insert
    ARNTL (BMAL1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112480-plasmid-map-sequence-id-230516

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  6. pLP-hSox2-ires-gfp

    Plasmid
    #49391
    Purpose
    mammalian bicistronic expression of human Sox2 and EGFP
    Depositor
    Randall McKinnon
    Insert
    Sox2 (SOX2 Human)
    Use
    Cre/Lox; Acceptor vector
    Tags
    6xHN, EGFP, and IRES
    Expression
    Mammalian
    Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect t…
    Promoter
    CMV
    Available since
    Dec. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. pHEY1.1.0-gDNA

    Plasmid
    #112393
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HEY1
    Depositor
    Kevin White
    Insert
    HEY1 (HEY1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112393-plasmid-map-sequence-id-230239

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  8. pLPS-hAkt1-gfp

    Plasmid
    #49388
    Purpose
    mammalian expression of human Akt1 fused to EGFP
    Depositor
    Randall McKinnon
    Insert
    Akt1 (AKT1 Human)
    Use
    Cre/Lox; Acceptor vector
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    tga to cca at nt 1725 with respect to GenBank ID …
    Promoter
    CMV
    Available since
    Nov. 21, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pTFAP2C.1.0-gDNA

    Plasmid
    #113792
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFAP2C
    Depositor
    Kevin White
    Insert
    TFAP2C (TFAP2C Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    113792-plasmid-map-sequence-id-241260

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  10. pDDIT3.1.0-gDNA

    Plasmid
    #112396
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor DDIT3
    Depositor
    Kevin White
    Insert
    DDIT3 (DDIT3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112396-plasmid-map-sequence-id-230243

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  11. 4RG2

    Plasmid
    #64658
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    53BP1 (TP53BP1 Human)
    Use
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    April 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. BRPF1 (3MO8)

    Plasmid
    #28107
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    BRPF1:JMC014-E01:C44383 (BRPF1 Human)
    Use
    Tags
    His-tag with integrated TEV protease site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. 3JZN

    Plasmid
    #60123
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EED:APC056-C06:C32050 (EED Human, EED:APC056-C06:C32050)
    Use
    Tags
    6xHis and GST
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pDNR-hAkt1

    Plasmid
    #49387
    Purpose
    donor vector for cloning human Akt1 with 6xHN tag
    Depositor
    Randall McKinnon
    Insert
    Akt1 (AKT1 Human)
    Use
    Cre/Lox; Donor vector
    Tags
    6xHN (6 repeating His-Asn subunits)
    Expression
    Mutation
    tga to cca at nt 1725 with respect to GenBank ID …
    Promoter
    none
    Available since
    Dec. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pGag∆MA

    Plasmid
    #212632
    Purpose
    Expression vector for MLV Gag with truncated MA domain
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of MA domain (amino acids 2-215)
    Promoter
    CAG
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    212632-plasmid-map-sequence-id-454202

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  17. pLV_CAG_Gag∆MA

    Plasmid
    #212633
    Purpose
    Lentivirus vector to express MLV Gag with truncated MA domain
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of MA domain (amino acids 2-215)
    Promoter
    CAG
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    212633-plasmid-map-sequence-id-422303

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  20. DCAF1:PBC020-D05:C246337

    Plasmid
    #183974
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    DCAF1:PBC020-D05:C246337 (DCAF1 Human)
    Use
    Baculovirus expresssion
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    May 10, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    183974-plasmid-map-sequence-id-361427

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Showing: 1341 - 1360 of 29406 results