We narrowed to 1,601 results for: tef

1. pRPL28(K55M) SpHIS5 (pNTI666)

   Plasmid

   #115436

   Purpose

   Expresses K55M RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Expression

   Yeast

   Mutation

   K55M; MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. STAGR_SAMScaffold_mU6

   Plasmid

   #102845

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert SAM_mU6
   

   Use

   Pcr template for stagr inserts

   Promoter

   mU6

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCT

   Plasmid

   #60620

   Purpose

   Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Inserts

   iCas9
   tracrRNA
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Yeast

   Mutation

   changed Aspartate 147 to Tyrosine, Proline 411 to…

   Promoter

   RPR1p and TEF1p

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYSD153

   Plasmid

   #212929

   Purpose

   Encodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Expression

   Yeast

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYSD141

   Plasmid

   #212928

   Purpose

   Encodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Expression

   Yeast

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAFMW-AthHEN1

   Plasmid

   #104980

   Purpose

   Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

   Insert

   Hen1 (HEN1 Mustard Weed)
   

   Tags

   FLAG Myc

   Expression

   Insect

   Promoter

   Actin5C

   Available Since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPMVAugw

   Plasmid

   #98300

   Purpose

   Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   TEFM1EfmvaE>TEBS1
   

   Expression

   Yeast

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTXB3 AurA

   Plasmid

   #68270

   Purpose

   bacterial expression of human AurA

   Depositor

   Stefano Ferrari

   Article

   Krystyniak et al Oncogene. 2006 Jan 19;25(3):338-48.

   Insert

   AurA (AURKA Human)
   

   Tags

   Mxe intein/chitin binding domain

   Expression

   Bacterial

   Mutation

   See depositor comment below

   Promoter

   T7

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

9. Flag-FOXN3

   Plasmid

   #153139

   Purpose

   Expression of Flag tagged human FOXN3 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXN3 (FOXN3 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHCas9-Nours

    Plasmid

    #107733

    Purpose

    Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

    Depositor

    Sheng Yang

    Article

    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Human optimized

    Promoter

    TEF1

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. STAGR_SAMScaffold_hH1

    Plasmid

    #102847

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert SAM_hH1
    

    Use

    Pcr template for stagr inserts

    Promoter

    U6

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMG

    Plasmid

    #138264

    Purpose

    Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Inserts

    eGFP flanked by iCas9 sites
    mCherry
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    Tef1

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)MBII-85-5'/3'ss mut

    Plasmid

    #67645

    Purpose

    MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

    Insert

    MBII85 snoRNA
    

    Expression

    Mammalian

    Mutation

    5' and 3' splice sites mutations

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPR01-FnCpf1

    Plasmid

    #176529

    Purpose

    Nuclease Cpf1 adapted for Aspergillus species

    Depositor

    Kusol Pootanakit

    Article

    Abdulrachman et al BMC Biotechnol. 2021 Feb 11;21(1):15. do

    Insert

    FnCpf1
    

    Use

    CRISPR; Fungal expression

    Promoter

    TEF1

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. STAGR_SAMScaffold_h7SK

    Plasmid

    #102846

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert SAM_h7SK
    

    Use

    Pcr template for stagr inserts

    Promoter

    H7SK

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR4_SNORD27 probe

    Plasmid

    #73069

    Purpose

    antisense probe for detection of human SNORD27

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD27 (SNORD2 Human)
    

    Use

    In vitro transcription

    Promoter

    t7

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)MBII-85-3'ss mut

    Plasmid

    #67644

    Purpose

    MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 3' splice site was mutated to consensus sequences.

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

    Insert

    MBII85 snoRNA
    

    Expression

    Mammalian

    Mutation

    3' splice site muattion

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYG026

    Plasmid

    #65328

    Purpose

    reporter plasmids used in YeastFab work, in which promoters could be inserted just upstream YFP(YEVenus) to drive its expression, also contains an mCherry gene driven by TEF2 promoter.

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    ccdB
    

    Use

    Unspecified

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. E547* FL USP7

    Plasmid

    #229001

    Purpose

    E547-mutant of human USP7

    Depositor

    Irina Bezsonova

    Insert

    USP7 1-546 (USP7 Human)
    

    Tags

    6xHis cleavable with thrombin

    Expression

    Bacterial

    Mutation

    E547 mutated to stop codon

    Promoter

    T7

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWR58

    Plasmid

    #202795

    Purpose

    integrating bioluminescene reporter for Cas9 activity in S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    P-TDH3/coCBG(first portion)-YUM1
    P-TEF1/ShBle/T-ACT1
    YUM1-coCBG(last portion)/T-ADH2
    URA3 and flanking sequence
    

    Expression

    Yeast

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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