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AiP15291 - pAAV-AiE0710m-minBG-iCre(R297T)-BGHpA (Alias: CN5291)

Plasmid

#230510

Purpose

AiE0710m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1375 - pAAV-AiE2146m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230634

Purpose

AiE2146m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-NRAS(G12D)-Neo

Plasmid

#232953

Purpose

Expresses mutant form of NRAS

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

NRAS(G12D)-Neo (NRAS Human)

Use

Lentiviral

Mutation

G12D

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1379 - pAAV-AiE2150m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230531

Purpose

AiE2150m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-TVA-mCherry

Plasmid

#38044

Depositor

Naoshige Uchida

Article

Watabe-Uchida et al Neuron. 2012 Jun 7;74(5):858-73.

Insert

Avian sarcoma and leukemia virus receptor 950 (CD320 Synthetic, quail)

Use

AAV and Cre/Lox

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pDAS12125_PEAR-GFP

Plasmid

#177178

Purpose

PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA C12 ecDHFR DD YFP HA

Plasmid

#192815

Purpose

Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

Depositor

John Hulleman

Article

Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

Insert

E. coli dihydrofolate reductase

Tags

enhanced yellow fluorescent protein and hemagglut…

Expression

Mammalian

Mutation

W74R/T113S/E120D/Q146L

Promoter

CMV

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pH2B-mNG2(11)

Plasmid

#206043

Purpose

Plasmid expressing a H2B-mNG2(11) fusion in mammalian cells for complementation with the mNG2(1-10) fragment.

Depositor

Alisa Piekny

Article

Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

Insert

H2B gene fused to the mNG2(11) fragment

Use

Synthetic Biology

Tags

H2B-mNG2(11)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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TALED_Left-ND1-AD-E1347A

Plasmid

#183894

Purpose

Expresses TALED_Left-ND1-AD-E1347A in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

TALED for ND1 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1_SPTLC1_WT

Plasmid

#181920

Purpose

3rd generation, Inducible lentiviral plasmid for expression of human wildtype SPTLC1

Depositor

Christian Metallo

Article

Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10

Insert

Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), (SPTLC1 Human)

Use

Lentiviral; Mammalian expression, , doxycycline i…

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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