Skip to main content
Addgene
Showing: 2721 - 2760 of 11043 results

  2. pWL502-crRNA

    Plasmid
    #174383
    Purpose
    The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    a crRNA targeting the template strand
    Use
    CRISPR; Archaeal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PhaR promoter
    Available since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174383-plasmid-map-sequence-id-343840

    Enlarge

  6. LC501: pMVP (L3-L2) mCherry + polyA

    Plasmid
    #121765
    Purpose
    pMVP L3-L2 entry plasmid, contains mCherry + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    mCherry + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121765-plasmid-map-sequence-id-237249

    Enlarge

  14. KP201: pMVP (L5-L4) eGFP-P2A

    Plasmid
    #121708
    Purpose
    pMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    eGFP-P2A (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121708-plasmid-map-sequence-id-237057

    Enlarge

  16. KP301: pMVP (L5-L4) mCherry-P2A

    Plasmid
    #121710
    Purpose
    pMVP L5-L4 entry plasmid, contains mCherry-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term mCherry linked by P2A to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    mCherry-P2A (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121710-plasmid-map-sequence-id-237060

    Enlarge

  19. p3E-rev_U6_MCS

    Plasmid
    #109769
    Purpose
    Multisite gateway vector for 3' expression of a guide RNA from the U6 promoter (cassette in the reverse orientation). Contains BseRI sites for insertion of gRNA sequence
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    U6-gRNA cassette
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109769-plasmid-map-sequence-id-224177

    Enlarge

  25. E1_pENTR_L1-L4_NI_G1397-DddtoxA-C

    Plasmid
    #171726
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    171726-plasmid-map-sequence-id-338715

    Enlarge

  26. E3_pENTR_L3-L2_NG_G1397-DddtoxA-C

    Plasmid
    #171734
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171734-plasmid-map-sequence-id-338634

    Enlarge

  27. E3_pENTR_L3-L2_NG_G1397-DddtoxA-N

    Plasmid
    #171735
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:
    Insert
    C- and N termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171735-plasmid-map-sequence-id-338620

    Enlarge

  28. E1_pENTR_L1-L4_NI_G1397-DddtoxA-N

    Plasmid
    #171727
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171727-plasmid-map-sequence-id-338618

    Enlarge

  29. E1_pENTR_L1-L4_NN_G1397-DddtoxA-N

    Plasmid
    #171731
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171731-plasmid-map-sequence-id-338617

    Enlarge

  30. E1_pENTR_L1-L4_NN_G1397-DddtoxA-C

    Plasmid
    #171730
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171730-plasmid-map-sequence-id-338458

    Enlarge

  34. p3E-FAPP1

    Plasmid
    #109555
    Purpose
    Multisite gateway vector for 3' tagging with the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4P
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3).
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109555-plasmid-map-sequence-id-222033

    Enlarge

  36. KP501: pMVP (L5-L4) eYFP-P2A

    Plasmid
    #121714
    Purpose
    pMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    eYFP-P2A (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121714-plasmid-map-sequence-id-237068

    Enlarge

  38. actc1b-mKate2-LactC2

    Plasmid
    #109499
    Purpose
    Muscle specific zebrafish expression of mKate2 fused to the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS)
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    actc1b-mKate2-LactC2
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109499-plasmid-map-sequence-id-219966

    Enlarge

  39. pWL502-PAM

    Plasmid
    #174382
    Purpose
    Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    PAM-spacer
    Use
    CRISPR; Archaeal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174382-plasmid-map-sequence-id-344136

    Enlarge

Showing: 2721 - 2760 of 11043 results