We narrowed to 4,495 results for: Ado

1. pAC1-pCR8-dCas9VP48

   Plasmid

   #48214

   Purpose

   dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP48 activation domain
   

   Use

   CRISPR; Gateway donor

   Tags

   HA Tag and VP48

   Expression

   Mutation

   D10A;H840A

   Promoter

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PX458_GATAD1_2

   Plasmid

   #104043

   Purpose

   Encodes gRNA for 3' target of human GATAD1 along with Cas9 with 2A GFP

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   GATAD1 gRNA (GATAD1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PX458_GATAD1_1

   Plasmid

   #104042

   Purpose

   Encodes gRNA for 3' target of human GATAD1 along with Cas9 with 2A GFP

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   GATAD1 gRNA (GATAD1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-BE3-2X

   Plasmid

   #110835

   Purpose

   CMV expression vector for BE3-2X construct (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3-2X
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2 tandem NLS sequences in the XTEN linker

   Promoter

   CMV

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHL417

   Plasmid

   #62822

   Purpose

   Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::micC::Asp terminator + pLtetO-1::RBS (st7) hfq::T1 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Inserts

   gfp
   micC leader
   hfq leader
   

   Use

   Synthetic Biology

   Tags

   ompC leader and st7 RBS

   Expression

   Bacterial

   Mutation

   Promoter

   pLlacO-1 and pLtetO-1

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pYO1124

   Plasmid

   #235725

   Purpose

   Expression of human UVRAG (1-464) fused to human ATG14L BATS (413-492) in mammalian cells

   Depositor

   Roger Williams

   Article

   Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

   Insert

   UVRAG(1-464) fused to ATG14L BATS domain (413-492)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJB180

   Plasmid

   #235737

   Purpose

   Expression of mCherry- human RAB1A Q70L in mammalian cells

   Depositor

   Roger Williams

   Article

   Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

   Insert

   RAB1A (RAB1A Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Q70L

   Promoter

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYO1296

   Plasmid

   #235710

   Purpose

   Expression of mCherry-human RAB5A Q79L in mammalian cells

   Depositor

   Roger Williams

   Article

   Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

   Insert

   RAB5 (RAB5A Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Q79L, aa 2-215

   Promoter

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLJC2-Blast-DHFR-3xFLAG

   Plasmid

   #233892

   Purpose

   lentiviral overexpression vector for DHFR

   Depositor

   Jason Cantor

   Article

   Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

   Insert

   DHFR (DHFR Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLJC2-Blast-DHFR

    Plasmid

    #233893

    Purpose

    lentiviral overexpression vector for DHFR

    Depositor

    Jason Cantor

    Article

    Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

    Insert

    DHFR (DHFR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYO1262

    Plasmid

    #235727

    Purpose

    Protein expression of His6-RAB1A Q70L 1-203 in bacteria

    Depositor

    Roger Williams

    Article

    Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

    Insert

    RAB1A (RAB1A Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Q70L, aa 1-203

    Promoter

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FAM177A1-Halo

    Plasmid

    #224591

    Purpose

    For expression of FAM177A1-Halo in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

    Insert

    FAM177A1 (FAM177A1 Human)
    

    Use

    Tags

    HALO

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLentiCRISPR-v1-sgMTHFD1

    Plasmid

    #217436

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human MTHFD1

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA targeting MTHFD1 (MTHFD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLJC2-TK2-3xFLAG

    Plasmid

    #217427

    Purpose

    Lentiviral overexpression of human TK2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    TK2 (TK2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Insert sequence is a codon-optimized gBLOCK (IDT)

    Promoter

    CMV

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLentiCRISPR-v1-sgTYMS

    Plasmid

    #217430

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human TYMS

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA targeting TYMS (TYMS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLentiCRISPR-v1-sgTK2_4

    Plasmid

    #217431

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA 4 targeting TK2 (TK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiCRISPR-v1-sgTK2_5

    Plasmid

    #217432

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA 5 targeting TK2 (TK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLentiCRISPR-v1-sgSHMT2

    Plasmid

    #217435

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human SHMT2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA targeting SHMT2 (SHMT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

    Plasmid

    #200792

    Purpose

    Mammalian expression of CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

    Depositor

    Alison Twelvetrees

    Article

    Smith et al bioRxiv 2024.12.20.629623

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    CLIPf and SNAPf, HisTag, FLAG

    Expression

    Mammalian

    Mutation

    Δ954-1027

    Promoter

    CMV & T7

    Available Since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

    Plasmid

    #200798

    Purpose

    Mammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

    Depositor

    Alison Twelvetrees

    Article

    Smith et al bioRxiv 2024.12.20.629623

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    CLIPf and SNAPf, HisTag, FLAG

    Expression

    Mammalian

    Mutation

    411-680

    Promoter

    CMV & T7

    Available Since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge