We narrowed to 5,218 results for: Ado
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Plasmid#228114PurposeExpress node 2 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 2, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
ExpressionMammalianPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM59
Plasmid#227655PurposepETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, Δ78–81)DepositorTagsH6-PreScission and PreScission-StrepExpressionBacterialMutation1-179 and amino acids 2-239, Δ78–81PromoterT7Available SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM54
Plasmid#227653PurposepETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, L132A)DepositorTagsH6-PreScission and PreScission-StrepExpressionBacterialMutation1-179 and amino acids 2-239, L132APromoterT7Available SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-PILATeS-RGD (200nM)
Plasmid#216716PurposeExpresses HaloTag-PILATeS-RGD with 200 nM BiT tag in bacteriaDepositorInsertHaloTag-PILATeS-RGD
Tags6xHis and HaloTagExpressionBacterialAvailable SinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-PILATeS-RGD (700pM)
Plasmid#216714PurposeExpresses HaloTag-PILATeS-RGD with 700 pM BiT tag in bacteriaDepositorInsertHaloTag-PILATeS-RGD
Tags6xHis and HaloTagExpressionBacterialAvailable SinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxEXPr6
Plasmid#218290PurposeIntroduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3DepositorInserthph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJQR3
Plasmid#218289PurposeIntroduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6DepositorInsertHO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036WExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylB-SCY_pET29b
Plasmid#215415PurposeExpression construct for NylB-SCY gene, codon optimized for expression in E. coliDepositorInsertNylB-SCY
ExpressionBacterialMutationR187S/F264C/D370YAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-LB-geneticin
Plasmid#213468PurposeVector to amplify the LB-geneticin fragment for transformation into TSI locus 1DepositorInsertLeft border of the TSI locus 1-split fragment of geneticin cassette
ExpressionBacterialAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2 RPS28 UTR1 SCR
Plasmid#203158PurposeRPS28 UTR luciferase assay plasmid (SCR UTR1, sites 1 and 2)DepositorInsertRPS28 UTR (RPS28 Human)
UseLuciferaseMutationscrambled the nucleotides in the two MIR-28 bindi…Available SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Combined γB2FL-FRET probe
Plasmid#209094PurposemTurquoise2- and mVenus-inserted PcdhgB2 full length (FL)DepositorAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Combined γB2ΔICD-FRET probe
Plasmid#209093PurposemTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)DepositorAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNHT7
Plasmid#186753PurposeA basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.DepositorTypeEmpty backboneUseSynthetic Biology; Cloning backbonePromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-Z-C-DiB-RM
Plasmid#168477PurposeC-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168475: pET11a-Z-N-DiB-RM to obtain DiB-RM‐split-Zip proteinDepositorInsertLeucine Zipper + C-fragment of DiB-RM
ExpressionBacterialPromoteraraBADAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-N-DiB-RM
Plasmid#168475PurposeN-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain DiB-RM‐split-Zip proteinDepositorInsertN-fragment of DiB-RM + Leucine Zipper
TagsHis-tagExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-rsAKARev(T/A)
Plasmid#166232PurposeExpresses reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant (rsAKARev(T/A)) in mammalian cellsDepositorInsertReversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant
ExpressionMammalianMutationchanged Threonine 506 in rsAKARev to AlaninePromoterCMVAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ELK1_iso1
Plasmid#135737PurposeDonor vector for 3' FLAG tag of human ELK1_iso1DepositorAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_MAF1_iso1
Plasmid#135732PurposeDonor vector for 3' FLAG tag of human MAF1_iso1DepositorAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX330_MAF1_iso1_2
Plasmid#135750PurposeEncodes gRNA for 3' target of human MAF1_iso1DepositorAvailable SinceJan. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX330_ELK1_iso1_1
Plasmid#135757PurposeEncodes gRNA for 3' target of human ELK1_iso1DepositorAvailable SinceJan. 27, 2020AvailabilityAcademic Institutions and Nonprofits only