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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 2,384 results for: LARGE;

Showing: 1361 - 1380 of 2384 results

  1. Bxb1_AJMA

    Plasmid
    #183760
    Purpose
    Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703
    Depositor
    Richard Davis
    Article
    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003
    Inserts
    ASAP2f
    jRCaMP1b
    miRFP703
    ASAP2f
    Use
    Tags
    Lck and NES
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183760-plasmid-map-sequence-id-460683

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  2. pIND(V5)EcoDam

    Plasmid
    #59201
    Purpose
    Mammalian DamID vector used to fuse gene of interest to V5-tagged E.coli Dam. Can also express Dam alone as negative control.
    Depositor
    Bas van Steensel
    Article
    Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep
    Type
    Empty backbone
    Use
    Damid
    Tags
    Dam (DNA adenine methyltransferase) and V5
    Expression
    Mammalian
    Mutation
    Promoter
    Heat Shock Minimal Promoter
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    59201-plasmid-map-sequence-id-247615

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  7. StrKDEL-IRES-ManII-mScarlet-i

    Plasmid
    #117274
    Purpose
    Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i
    Depositor
    David Stephens
    Article
    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180
    Inserts
    partial sequence of mannosidase II (Man2a1 Mouse)
    Streptavidin-KDEL
    Use
    Tags
    mScarlet-i
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV and IRES
    Available since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    117274-plasmid-map-sequence-id-233028

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  14. pRW004-SM-destination-proUBQ10-Cas9

    Plasmid
    #104438
    Purpose
    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    UBQ10
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104438-plasmid-map-sequence-id-201144

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  15. pEF005-sgRNA-shuffle-in

    Plasmid
    #104441
    Purpose
    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Insert
    sgRNA-transRNA transcription by U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104441-plasmid-map-sequence-id-201145

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Showing: 1361 - 1380 of 2384 results