We narrowed to 5,554 results for: donor

1. pHACK-GAL4>GAL80

   Plasmid

   #104874

   Purpose

   HACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.

   Depositor

   Christopher Potter

   Insert

   T2A-GAL80
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFETCh_ZNF219

   Plasmid

   #72367

   Purpose

   Donor vector for 3' FLAG tag of human ZNF219

   Depositor

   Richard M. Myers, Eric Mendenhall

   Insert

   ZNF219 Homology Arms (ZNF219 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFETCh_TGIF2

   Plasmid

   #72373

   Purpose

   Donor vector for 3' FLAG tag of human TGIF2

   Depositor

   Richard M. Myers, Eric Mendenhall

   Insert

   TGIF2 Homology Arms (TGIF2 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFETCh_ZNF146

   Plasmid

   #72359

   Purpose

   Donor vector for 3' FLAG tag of human ZNF146

   Depositor

   Richard M. Myers, Eric Mendenhall

   Insert

   ZNF146 Homology Arms (ZNF146 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. CRISPR plasmid

   Plasmid

   #236553

   Purpose

   Encodes Cas9 and two guideRNAs, one to cut the genome downstream of HSPA5 (BiP/GRP78) and one to linearize the DONOR plasmid.

   Depositor

   Pablo Rivera-Fuentes

   Insert

   Cas9
   

   Expression

   Mammalian

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-K40R-short-UBASH3A-Ntag

   Plasmid

   #195401

   Purpose

   Human K40R short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

   Depositor

   Patrick Concannon

   Insert

   UBASH3A (UBASH3A Human)
   

   Use

   Gateway donor vector

   Mutation

   K40R

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-K40R-short-UBASH3A-Ctag

   Plasmid

   #195400

   Purpose

   Human K40R short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

   Depositor

   Patrick Concannon

   Insert

   UBASH3A (UBASH3A Human)
   

   Use

   Gateway donor vector

   Mutation

   K40R

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFETCh_PBX2

   Plasmid

   #72356

   Purpose

   Donor vector for 3' FLAG tag of human PBX2

   Depositor

   Richard M. Myers, Eric Mendenhall

   Insert

   PBX2 (PBX2 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-N-Puro-mStayGold-ACTB

   Plasmid

   #227320

   Purpose

   Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCVL-UCOE-SFFV-TLR-MCV1

    Plasmid

    #117111

    Purpose

    Lentiviral vector encoding Traffic light reporter 2.0. Alternative name: pCVL-UCOE-SFFV-TLR-MCV1.

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    UCOE SFFV Traffic Light Reporter 2.0
    

    Use

    Lentiviral; Reporter

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-Blasticidin XLone-eGFP

    Plasmid

    #159754

    Purpose

    AAVS1 donor plasmid for targeted inducible eGFP expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    all-in-one tet-on system
    

    Use

    CRISPR and TALEN; Donor plasmid for targeted knoc…

    Expression

    Mammalian

    Promoter

    TRE3GS inducible promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

    Plasmid

    #196196

    Purpose

    homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
    

    Use

    Homology repair donor

    Tags

    V5-APEX2-mScarlet-I

    Available Since

    April 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. OCT4-eGFP-PGK-Puro

    Plasmid

    #31937

    Purpose

    Oct4 targeting donor vector

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Insert

    OCT4 (POU5F1 Human)
    

    Use

    Targeting donor

    Tags

    eGFP

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-mCherry-DMDEx23-eGFP

    Plasmid

    #211366

    Purpose

    Piggybac transposon plasmid for a DMD exon 23 skipping reporter (mCherry-DMDEx23)

    Depositor

    Ulrich Lächelt

    Article

    Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

    Insert

    mCherry-DMDEx23-eGFP
    

    Expression

    Mammalian

    Mutation

    mCherry interrupted by mdx dystrophin exon 23 bet…

    Promoter

    CAG

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA1

    Plasmid

    #117405

    Purpose

    SpyCas9 sgRNA 1 targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SpyCas9 sgRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221 MLK1 E179K

    Plasmid

    #60541

    Purpose

    Donor vector for human MLK1 E179K mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    MLK1 (MAP3K9 Human)
    

    Use

    Gateway donor vector

    Mutation

    E179K

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pDONR221 MLK1 D294A

    Plasmid

    #60542

    Purpose

    Donor vector for human MLK1 D294A mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    MLK1 (MAP3K9 Human)
    

    Use

    Gateway donor vector

    Mutation

    D294A

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA2

    Plasmid

    #117406

    Purpose

    SpyCas9 sgRNA 2 targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SpyCas9 sgRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1-puro-U6-AsCas12a-TLR-MCV1-gRNA

    Plasmid

    #117411

    Purpose

    AsCas12a-gRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    AsCas12a gRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1-Puro cTnT CCND2-T2A-ChloC-P2A-eGFP

    Plasmid

    #179840

    Purpose

    donor plasmid for cardiac-specific CCND2-T2A-Chloc-P2A-eGFP expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

    Insert

    CCND2 (CCND2 Human)
    

    Use

    CRISPR and TALEN; Donor plasmid

    Tags

    chloride-conducting ChR (ChloC) and eGFP

    Expression

    Mammalian

    Promoter

    Troponin T promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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