We narrowed to 5,293 results for: pcDNA3.1+

1. pcDNA3.1-Lari-BFP2-TSERex

   Plasmid

   #123311

   Purpose

   Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

   Depositor

   Yulong Li

   Article

   Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

   Insert

   Lari-BFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Tat101(AD8)-Flag

   Plasmid

   #115777

   Purpose

   pCMV-Tat101AD8-Flag allows the expression of Tat101(AD8) protein with a C-terminal flag-tag.

   Depositor

   Damian Purcell

   Article

   Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

   Insert

   HIV-1 Tat protein (AD8)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1/hChR2(H134R)-EYFP

   Plasmid

   #20940

   Purpose

   Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CMV

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-OTUB1 C91S-pcDNA3.1

   Plasmid

   #118210

   Purpose

   Expresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   OTUB1 (OTUB1 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   changed cysteine 91 to serine

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 SARS-CoV-2 S D614G

   Plasmid

   #158075

   Purpose

   Expresses SARS-CoV-2 spike protein with a D614G mutation

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-N-Myc_535-1102 USP7

   Plasmid

   #131246

   Purpose

   Mammalian expression of the five USP7 UBL domains with an N-terminal Myc tag.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Expresses USP7 amino acids 535-1102. This truncat…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 Hygro EYFP H148Q

   Plasmid

   #25873

   Depositor

   Peter Haggie

   Article

   Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

   Insert

   EYFP H148Q
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EYFP from pEYFP (Clontech) with H148Q mutation

   Promoter

   Available since

   Aug. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-TAT-1-101-FLAG

   Plasmid

   #35981

   Depositor

   Susana Valente

   Article

   Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

   Insert

   transactivating protein (tat )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   tat from HIV1 101 amino acids

   Promoter

   Available since

   Sept. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1+CMV-Ins-GLuc

   Plasmid

   #89929

   Purpose

   Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

   Depositor

   Melanie Cobb

   Article

   Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

   Insert

   human insulin-Gaussia-Luciferase (INS Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   DNA sequence for Gaussia luciferase was humanized…

   Promoter

   CMV

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-mas-GRK2RH-Nluc

    Plasmid

    #158605

    Purpose

    Plasmid expressing GRK2(RH)-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA3.1-nAChr-Alpha6-GFP

    Plasmid

    #50486

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Use

    Tags

    GFP fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Mutation

    Gly-Ala-Gly flexible linker flanking the GFP open…

    Promoter

    CMV

    Available since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pCDNA3.1-nAChr-beta3-YFP

    Plasmid

    #50484

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    YFP fusion in M3-M4 loop (after residue P379). O…

    Promoter

    CMV

    Available since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1-nAChr-Alpha6-mCherry

    Plasmid

    #50480

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Use

    Tags

    mCherry fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1/opto-b2AR-EYFP

    Plasmid

    #20948

    Purpose

    Mammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signaling

    Depositor

    Karl Deisseroth

    Article

    Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

    Insert

    opto-b2AR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1/Myc-His(-)-MSulf-2

    Plasmid

    #13008

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    mouse sulfatase 2 (Sulf2 Mouse)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-CarO-BFP2-TSERex

    Plasmid

    #124795

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    CarO-BFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-Mac-BFP2-TSERex

    Plasmid

    #124793

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    Mac-BFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-HNF1A-WT-Flag-UTR

    Plasmid

    #183237

    Purpose

    Expression of FLAG tagged HNF1A

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    HNF1 homeobox A (HNF1A Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-Clover-Prx2-mRuby2

    Plasmid

    #117906

    Purpose

    fluorescent reporter of cytosolic hydrogen peroxide

    Depositor

    Hadley Sikes

    Article

    Langford et al Nat Commun. 2018 Aug 7;9(1):3145. doi: 1

    Insert

    Clover-Prx2-mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-N-Myc_1-205 USP7

    Plasmid

    #131245

    Purpose

    Mammalian expression of the USP7 TRAF-like domain with an N-terminal Myc tag.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Expresses a mutant form of our pcDNA3.1-N-Myc_1-5…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge