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pMOD_A1710

Plasmid

#91039

Purpose

Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-TaCas9_H840A nickase

Use

CRISPR

Tags

Expression

Mutation

H840A

Promoter

ZmUbi

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTaCas9-D10A_5

Plasmid

#91170

Purpose

Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_D10A (nickase)

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTaCas9-H840A_6

Plasmid

#91173

Purpose

Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_H840A (nickase)

Use

CRISPR

Tags

Expression

Plant

Mutation

H840A

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAtCas9-dead_2

Plasmid

#91166

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A+H840A (dead)

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, H840A

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAtCas9-H840A_1

Plasmid

#91162

Purpose

Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_H840A (nickase)

Use

CRISPR

Tags

Expression

Plant

Mutation

H840A

Promoter

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_A0802

Plasmid

#91023

Purpose

Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_dead (D10A + H840A)

Use

CRISPR

Tags

Expression

Mutation

D10A, H840A

Promoter

AtUbi10

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAtCas9-dead_1

Plasmid

#91165

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A+H840A (dead)

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, H840A

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJG493

Plasmid

#91197

Purpose

WDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 control (gUbi1+gUbi8+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gUbi1+gUbi8+donor

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTaCas9_5

Plasmid

#91168

Purpose

Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAtCas9-H840A_2

Plasmid

#91163

Purpose

Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_H840A (nickase)

Use

CRISPR

Tags

Expression

Plant

Mutation

H840A

Promoter

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJG486

Plasmid

#91195

Purpose

WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi1+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nTaCas9_H840A+gUbi1+donor

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_C3106

Plasmid

#91101

Purpose

Module C, Promoter: FMV 34S, Gene: TALE-VPR backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TALE-VPR backbone with Esp3I ccdb cassette for repeat cloning

Use

TALEN

Tags

Expression

Mutation

Promoter

FMV 34S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTC393

Plasmid

#91221

Purpose

protoplast vector expressing gRNA24 targeting tomato ANT1 (with TREX2 expression)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting tomato ANT1

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTC363

Plasmid

#91211

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtU6 promoters)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_A0701

Plasmid

#91019

Purpose

Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_H840A nickase

Use

CRISPR

Tags

Expression

Mutation

H840A

Promoter

35S

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTC303

Plasmid

#91210

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAtCas9-dead_3

Plasmid

#91167

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A+H840A (dead)

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, H840A

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJG484

Plasmid

#91194

Purpose

WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi8+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nTaCas9_H840A+gUbi8+donor

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_C3306

Plasmid

#91102

Purpose

Module C, Promoter: FMV 34S, Gene: TALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning

Use

TALEN

Tags

Expression

Mutation

Promoter

FMV 34S

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_A1010

Plasmid

#91030

Purpose

Module A, Promoter: ZmUbi, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning

Use

TALEN

Tags

Expression

Mutation

Promoter

ZmUbi

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge