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Showing: 121 - 140 of 262 results

  1. pML325_GPR107-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227909
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    GPR107 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227909-plasmid-map-sequence-id-452501

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  7. GPR180-COMP-blac-flag-his

    Plasmid
    #110955
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    PF3D7_1213500 (PF3D7_1213500 )
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    110955-plasmid-map-sequence-id-221974

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  9. AiP937-Gpr139-IRES2-FlpO-WPRE-neo

    Plasmid
    #181768
    Purpose
    targeting vector
    Depositor
    Tanya Daigle
    Insert
    Gpr139 homology arms, FlpO
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    181768-plasmid-map-sequence-id-356727

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  13. MRGPRF-DuET

    Plasmid
    #213342
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MRGPRF (MRGPRF Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213342-plasmid-map-sequence-id-430870

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  16. pPMS-1034

    Plasmid
    #232283
    Purpose
    Protein Expression
    Depositor
    Jeremy Nathans
    Insert
    GPR124 (Gpr124 Mouse)
    Use
    Tags
    3X HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232283-plasmid-map-sequence-id-461126

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  17. pPMS-932

    Plasmid
    #232277
    Purpose
    Protein Expression
    Depositor
    Jeremy Nathans
    Insert
    GPR125 with lower GC content near ATG (Gpr125 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Reduced GC content near ATG
    Promoter
    Available since
    Feb. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232277-plasmid-map-sequence-id-462380

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Showing: 121 - 140 of 262 results