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pKM394

Plasmid

#134211

Purpose

Baculovirus expression of CENP-N and CENP-L

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-N-his-CENP-L

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pKM410

Plasmid

#134212

Purpose

Baculovirus expression of CENP-T and CENP-W

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-T+W

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKM463

Plasmid

#134214

Purpose

Baculovirus expression of CENP-U and CENP-Q

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENP-U + Q

Expression

Insect

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pKM377

Plasmid

#134210

Purpose

Baculovirus expression of CENP-HKM and His-CENP-I

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-H/K/M untagged + his-I

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJM111

Plasmid

#134162

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC 295-C (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTK158_mCherry-4.1G-deltaCTD-hardened

Plasmid

#46360

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G (EPB41L2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Deleted amino acids 886-1005, and modified for si…

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pIC389

Plasmid

#37387

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

LGN (GPSM2 Human)

Use

Retroviral

Tags

GFP, S-tag, and TEV

Expression

Mammalian

Mutation

deleted amino acid 1-364 and 524

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pKM499

Plasmid

#134216

Purpose

Baculovirus expression of CENP-U CENP-Q CENP-OP

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENPU + CENPQ+ CENPOP

Expression

Insect

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pKM744

Plasmid

#134225

Purpose

Baculovirus expression of CENP-T and CENP-W

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENP T + CENP W

Expression

Insect

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pKM149

Plasmid

#134178

Purpose

Bacterial expression of his-CENP-C 510-end

Depositor

Iain Cheeseman

Article

McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

Insert

his-CENP-C 510-end (CENPC Human)

Expression

Bacterial

Mutation

aa 510-end

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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AP-p003

Plasmid

#31494

Depositor

David Wang

Article

Gaynor et al PLoS Pathog. 2007 May 4. 3(5):e64.

Insert

Half genome of WU polyomavirus

Expression

Bacterial

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pTK157_mCherry-4.1G-CTD

Plasmid

#46359

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G C-terminal domain (EPB41L2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

C-terminal domain from 886-1005 amino acids

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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AP-p002

Plasmid

#31493

Depositor

David Wang

Article

Gaynor et al PLoS Pathog. 2007 May 4. 3(5):e64.

Insert

Half genome of WU polyomavirus

Expression

Bacterial

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pNL3.2.CMV.3UTR-TH.NLuc-PEST.Hygro

Plasmid

#209952

Purpose

In mammalian cells expresses NanoLuc mRNA with the TH in the 3'-UTR. Also expresses a hygromycin resistance marker.

Depositor

Kevin Weeks

Article

Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38

Inserts

NanoLuc
Hygromycin resistance marker

Expression

Mammalian

Promoter

CMV and SV40

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pNL3.2.CMV.5UTR-TH.NLuc-PEST.Hygro

Plasmid

#209953

Purpose

In mammalian cells expresses NanoLuc mRNA with the TH in the 5'-UTR. Also expresses a hygromycin resistance marker.

Depositor

Kevin Weeks

Article

Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38

Inserts

NanoLuc
Hygromycin resistance marker

Expression

Mammalian

Promoter

CMV and SV40

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pKC530 pBABE EGFP-ZNF335

Plasmid

#192698

Purpose

Retroviral transgene expression of human ZNF335

Depositor

Iain Cheeseman

Article

Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

Insert

ZNF335 (ZNF335 Human)

Use

Retroviral

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pZS181

Plasmid

#177637

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

HaloTag

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pZS212

Plasmid

#177639

Purpose

In vitro transcription of Patiria miniata Lamp1

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Lamp1

Use

In vitro transcription

Tags

mCherry

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pZS217

Plasmid

#177640

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pZS221

Plasmid

#177643

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Mutation

K57A, K58A

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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