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pDY1440 STITCHR GFP NOLC1 payload

Plasmid

#234829

Purpose

GFP cargo with 50bp NOLC homology arms

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

copGFP

Use

Tags

Expression

Mutation

Promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDY1656 STITCHR Gluc AAVS1 payload

Plasmid

#234827

Purpose

Gluc exon 2 with 50bp AAVS1 homology arms

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

Gluc exon 2

Use

Tags

Expression

Mutation

Promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDY1513 AAVS1 sgRNA

Plasmid

#234832

Purpose

sgRNA for AAVS1 STITCHR insertion

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

AAVS1 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLS-SceI

Plasmid

#137725

Purpose

Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

Depositor

Nadav Ahituv

Article

Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRXRα

Plasmid

#135910

Purpose

Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 Promoter

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Insert

Human retinoid X receptor- alpha (RXRA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLS-SV40-mP-EGFP

Plasmid

#137724

Purpose

Lentivirus based EGFP expression vector

Depositor

Nadav Ahituv

Article

Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

Insert

SV40 enhancer

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRARα.hRXRα

Plasmid

#135411

Purpose

Human Retinoic Acid Receptor-alpha and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Inserts

Human retinoic acid receptor-alpha
Human retinoid X receptor-alpha

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and SV40

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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JT113-pETDuet1-(R)-hREV3L

Plasmid

#64872

Purpose

bacterial expression of human REV3L

Depositor

Richard Wood

Article

Tomida et al Nucleic Acids Res. 2015 Jan;43(2):1000-1

Insert

REV3L (REV3L Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRARγ.hRXRα

Plasmid

#135416

Purpose

Human Retinoic Acid Receptor-gamma and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Inserts

Human retinoic acid receptor-gamma
Human retinoid X receptor-alpha

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and SV40

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRARβ.hRXRα

Plasmid

#135415

Purpose

Human Retinoic Acid Receptor-beta and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Inserts

Human retinoic acid receptor-beta
Human retinoid X receptor-alpha

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and SV40

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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sIL6R PLAK1

Plasmid

#161519

Purpose

Expresses soluble IL6 receptor in mammalian cells.

Depositor

Yoav Shaul

Article

Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

Insert

soluble IL-6 receptor

Use

Lentiviral

Tags

3X Flag

Expression

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-hCYP26A1P-E4-mCherry

Plasmid

#135478

Purpose

Human short form promoter of human gene in pGL3-Basic-mCherry plasmid vector (Namely, E4.2)

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Insert

mCherry Open reading Frame

Use

Unspecified

Tags

Expression

Mutation

Promoter

Short from promoter of human CYP26A1 (312 bp)

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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∆C' terminal-DPYSL2-pLJC2

Plasmid

#189867

Purpose

Expresses human DPYSL2 (CRMP2) lacking the C' terminus (491-572 aa), in mammalian cells

Depositor

Yoav Shaul

Article

Abu Rmaileh et al J Cell Biol. 2022 Jul 4;221(7). pii: 213

Insert

ΔC-DPYSL2

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Mutation

deleted the C'terminus of DPYSL2 (491-572 aa)

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pRSETa-Amrose v3

Plasmid

#79539

Purpose

pRSETa (Invitrogen) plasmid expressing Amrose variant 3

Depositor

Randolph Caldwell

Article

Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

Insert

Amrose v3

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-RNAPII-A2-14-HA

Plasmid

#160689

Purpose

Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains an S-to-A mutation in position 2 of 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

Depositor

Jeffry Corden

Article

Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

Insert

RNA polymerase II CTD

Use

Tags

Expression

Bacterial

Mutation

Contains an S-to-A mutation in position 2 of 14 o…

Promoter

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETa-Amrose v2

Plasmid

#79538

Purpose

pRSETa (Invitrogen) plasmid expressing Amrose variant 2

Depositor

Randolph Caldwell

Article

Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

Insert

Amrose v2

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc

Plasmid

#234826

Purpose

STITCHR R2Tocc editor

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

nCas9h840a-xten-R2Tocc

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-basic-hRARβP-FL-luciferase

Plasmid

#135447

Purpose

Human full-length promoter of retinoic acid receptor-beta gene in pGL3-Basic-luciferase plasmid vector

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Insert

Full-length promoter of human retinoic acid receptor-beta gene

Use

Tags

Expression

Mutation

Promoter

Human RARβ promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pKG-GW1

Plasmid

#203163

Purpose

Yeast expression vector; To express proteins under control of TDH3 promoter and terminator using leucine selection

Depositor

William Sessa

Article

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pUT1159-HQ858703

Plasmid

#27749

Depositor

Erich Grotewold, John Gray

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

AUX/IAA transcription factor from DV030103

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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